{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang J"],"funding":["University of North Carolina Wilmington","National Institutes of Health","The University of North Carolina at Chapel Hill"],"pagination":["100864"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9800194"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["36"],"pubmed_abstract":["Purpose
The underlying mechanism for radiation as a potentiator of immune checkpoint inhibition (ICI) is unclear. We developed a novel murine model to investigate the effects of post-irradiation intratumoral heterogeneity (ITH) on response to ICI.Experimental design
Parental mouse melanoma B16F10 cells were irradiated in vitro (5Gy x 3 fractions), then an a priori determined number of resulting colonies were implanted in C57BL/6J immunocompetent mice creating syngeneic models of unirradiated (parental) and irradiated tumors with low (irradiated-L) and high (irradiated-H) ITH. Mice were treated with placebo, α-PD-L1, α-CTLA-4 or dual ICI. Murine tumors underwent whole exome sequencing (WES). Clinically correlated paired pre- and post-irradiation patient rectal adenocarcinoma"],"journal":["Neoplasia (New York, N.Y.)"],"pubmed_title":["Post-irradiation intratumoral heterogeneity modulates response to immune checkpoint inhibition therapy in a murine melanoma model."],"pmcid":["PMC9800194"],"funding_grant_id":["U54-CA151652","R01CA178748"],"pubmed_authors":["Zhang J","Wagner KT","Kim IJ","Knape NM","Wang J","Li L","Marron D","Zhao Y","Zhang T","Guo G","Wang AZ","Qu Y","Sud S"],"additional_accession":[]},"is_claimable":false,"name":"Post-irradiation intratumoral heterogeneity modulates response to immune checkpoint inhibition therapy in a murine melanoma model.","description":"Purpose
The underlying mechanism for radiation as a potentiator of immune checkpoint inhibition (ICI) is unclear. We developed a novel murine model to investigate the effects of post-irradiation intratumoral heterogeneity (ITH) on response to ICI.Experimental design
Parental mouse melanoma B16F10 cells were irradiated in vitro (5Gy x 3 fractions), then an a priori determined number of resulting colonies were implanted in C57BL/6J immunocompetent mice creating syngeneic models of unirradiated (parental) and irradiated tumors with low (irradiated-L) and high (irradiated-H) ITH. Mice were treated with placebo, α-PD-L1, α-CTLA-4 or dual ICI. Murine tumors underwent whole exome sequencing (WES). Clinically correlated paired pre- and post-irradiation patient rectal adenocarcinoma","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-26T00:27:54.615Z","creation":"2025-04-06T09:41:56.542Z"},"accession":"S-EPMC9800194","cross_references":{"pubmed":["36571944"],"doi":["10.1016/j.neo.2022.100864"]}}