{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28"],"submitter":["Penza V"],"pubmed_abstract":["Mengovirus is an oncolytic picornavirus whose broad host range allows for testing in immunocompetent cancer models. Two pathogenicity-ablating approaches, polycytidine (polyC) tract truncation and microRNA (miRNA) targets insertion, eliminated the risk of encephalomyocarditis. To investigate whether a polyC truncated, miRNA-detargeted oncolytic Mengovirus might be boosted, we partially or fully rebuilt the polyC tract into the 5' noncoding region (NCR) of polyC-deleted (MC<sub>0</sub>) oncolytic constructs (NC) carrying miRNA target (miRT) insertions to eliminate cardiac/muscular (miR-133b and miR-208a) and neuronal (miR-124) tropisms. PolyC-reconstituted viruses (MC<sub>24</sub>-NC and MC<sub>37</sub>-NC) replicated <i>in vitro</i> and showed the expected tropism restrictions, but reduced"],"journal":["Molecular therapy oncolytics"],"pagination":["15-30"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9800256"],"repository":["biostudies-literature"],"pubmed_title":["Polycytidine tract deletion from microRNA-detargeted oncolytic Mengovirus optimizes the therapeutic index in a murine multiple myeloma model."],"pmcid":["PMC9800256"],"pubmed_authors":["Penza V","Schulze AJ","Nace RA","Russell SJ","Maroun JW"],"additional_accession":[]},"is_claimable":false,"name":"Polycytidine tract deletion from microRNA-detargeted oncolytic Mengovirus optimizes the therapeutic index in a murine multiple myeloma model.","description":"Mengovirus is an oncolytic picornavirus whose broad host range allows for testing in immunocompetent cancer models. Two pathogenicity-ablating approaches, polycytidine (polyC) tract truncation and microRNA (miRNA) targets insertion, eliminated the risk of encephalomyocarditis. To investigate whether a polyC truncated, miRNA-detargeted oncolytic Mengovirus might be boosted, we partially or fully rebuilt the polyC tract into the 5' noncoding region (NCR) of polyC-deleted (MC<sub>0</sub>) oncolytic constructs (NC) carrying miRNA target (miRT) insertions to eliminate cardiac/muscular (miR-133b and miR-208a) and neuronal (miR-124) tropisms. PolyC-reconstituted viruses (MC<sub>24</sub>-NC and MC<sub>37</sub>-NC) replicated <i>in vitro</i> and showed the expected tropism restrictions, but reduced","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2025-04-04T12:37:29.288Z","creation":"2025-04-04T12:37:29.288Z"},"accession":"S-EPMC9800256","cross_references":{"pubmed":["36619293"],"doi":["10.1016/j.omto.2022.11.006"]}}