biostudies-literatureFilon MJUniversity of Wisconsin Carbone Cancer CenterU.S. Department of DefenseNIH Clinical CenterNCI NIH HHSAmerican Urological Association Foundation1547-1557https://www.ebi.ac.uk/biostudies/studies/S-EPMC9804183biostudies-literatureUnknown82(16)<h4>Background</h4>Androgen deprivation therapy (ADT) has been the standard of care for advanced hormone-sensitive prostate cancer (PC), yet tumors invariably develop resistance resulting in castrate-resistant PC. The acute response of cancer cells to ADT includes apoptosis and cell death, but a large fraction remains arrested but viable. In this study, we focused on intensively characterizing the early metabolic changes that result after ADT to define potential metabolic targets for treatment.<h4>Methods</h4>A combination of mass spectrometry, optical metabolic imaging which noninvasively measures drug responses in cells, oxygen consumption rate, and protein expression analysis was used to characterize and block metabolic pathways over several days in multiple PC cell lines with variable The ProstateProstate cancer cells demonstrate unique metabolism and substrate adaptability acutely after androgen deprivation therapy.PMC9804183R01 CA185747R37 CA22PCRP 150221Tariq KhemeesR01 CA211082P30 CA014520R01 CA205101Khemees TASkala MCJarrard DFYang BGillette AAFilon MJfalseProstate cancer cells demonstrate unique metabolism and substrate adaptability acutely after androgen deprivation therapy.<h4>Background</h4>Androgen deprivation therapy (ADT) has been the standard of care for advanced hormone-sensitive prostate cancer (PC), yet tumors invariably develop resistance resulting in castrate-resistant PC. The acute response of cancer cells to ADT includes apoptosis and cell death, but a large fraction remains arrested but viable. In this study, we focused on intensively characterizing the early metabolic changes that result after ADT to define potential metabolic targets for treatment.<h4>Methods</h4>A combination of mass spectrometry, optical metabolic imaging which noninvasively measures drug responses in cells, oxygen consumption rate, and protein expression analysis was used to characterize and block metabolic pathways over several days in multiple PC cell lines with variable 2022-01-01T00:00:00Z2022 Dec2025-04-05T12:10:17.754Z2025-04-05T12:10:17.754ZS-EPMC98041833598083110.1002/pros.24428