biostudies-literatureGarcia-Montojo MALS Association782-792https://www.ebi.ac.uk/biostudies/studies/S-EPMC9805205biostudies-literatureUnknown92(5)<h4>Objectives</h4>Reactivation of HERV-K(HML-2) has been found in subsets of individuals with amyotrophic lateral sclerosis (ALS). This study examines the antibody response against HML-2 in ALS and analyzes its clinical relevance.<h4>Methods</h4>Antibodies to HML-2 envelope (env) were analyzed using a peptide array for epitope mapping and by a peptide enzyme-linked immunosorbent assay (ELISA) in 242 healthy donors, and 243 ALS and 85 multiple sclerosis (MS) individuals. Extracellular levels of HML-2 were analyzed by digital polymerase chain reaction (PCR).<h4>Results</h4>Antibodies in the sera of ALS individuals recognized more HML-2 env peptides compared to healthy controls (p < 0.0001). ALS individuals had higher levels of HML-2 than healthy donors (p = 0.02) and higher antibody levels Annals of neurologyAntibody Response to HML-2 May Be Protective in Amyotrophic Lateral Sclerosis.PMC980520520‐SI‐559Fathi SSimula ERJohnson KBerry JDNath ACudkowicz MSechi LAGarcia-Montojo MGhosal ANorato GMcMahan CElkahloun AJames TJensen PfalseAntibody Response to HML-2 May Be Protective in Amyotrophic Lateral Sclerosis.<h4>Objectives</h4>Reactivation of HERV-K(HML-2) has been found in subsets of individuals with amyotrophic lateral sclerosis (ALS). This study examines the antibody response against HML-2 in ALS and analyzes its clinical relevance.<h4>Methods</h4>Antibodies to HML-2 envelope (env) were analyzed using a peptide array for epitope mapping and by a peptide enzyme-linked immunosorbent assay (ELISA) in 242 healthy donors, and 243 ALS and 85 multiple sclerosis (MS) individuals. Extracellular levels of HML-2 were analyzed by digital polymerase chain reaction (PCR).<h4>Results</h4>Antibodies in the sera of ALS individuals recognized more HML-2 env peptides compared to healthy controls (p < 0.0001). ALS individuals had higher levels of HML-2 than healthy donors (p = 0.02) and higher antibody levels 2022-01-01T00:00:00Z2022 Nov2026-05-28T03:54:40.584Z2025-02-19T02:37:49.809ZS-EPMC98052053605395110.1002/ana.26466