biostudies-literatureLi RKey Program of Sichuan Science and Technology DepartmentNational Natural Science Foundation of China113https://www.ebi.ac.uk/biostudies/studies/S-EPMC9805692biostudies-literatureUnknown53(1)Listeriolysin O (LLO) is the main virulence protein of Listeria monocytogenes (LM), that helps LM escape lysosomes. We previously found that the cellular immune response elicited by L.ivanovii (LI) is weaker than that elicited by LM. We speculated that this may be related to the function of ivanolysin O (ILO). Here, we constructed hemolysin gene deletion strain, LIΔilo, and a modified strain, LIΔilo::hly, in which ilo was replaced by hly. Prokaryotic transcriptome sequencing was performed on LI, LIΔilo, and LIΔilo::hly. Transcriptome differences between the three strains were compared, and genes and pathways with significant differences between the three strains were analyzed. Prokaryotic transcriptome sequencing results revealed the relationship of ilo to the ribosome, quorum sensing, andVeterinary researchHemolysin function of Listeria is related to biofilm formation: transcriptomics analysis.PMC98056922021YFQ0060315709242021YFS0005Ou QChen ZZhang YWang CTian SLi RLiu SLiang QfalseHemolysin function of Listeria is related to biofilm formation: transcriptomics analysis.Listeriolysin O (LLO) is the main virulence protein of Listeria monocytogenes (LM), that helps LM escape lysosomes. We previously found that the cellular immune response elicited by L.ivanovii (LI) is weaker than that elicited by LM. We speculated that this may be related to the function of ivanolysin O (ILO). Here, we constructed hemolysin gene deletion strain, LIΔilo, and a modified strain, LIΔilo::hly, in which ilo was replaced by hly. Prokaryotic transcriptome sequencing was performed on LI, LIΔilo, and LIΔilo::hly. Transcriptome differences between the three strains were compared, and genes and pathways with significant differences between the three strains were analyzed. Prokaryotic transcriptome sequencing results revealed the relationship of ilo to the ribosome, quorum sensing, and2022-01-01T00:00:00Z2022 Dec2026-07-14T16:59:01.698Z2025-04-04T03:42:11.752ZS-EPMC98056923658720610.1186/s13567-022-01124-y