biostudies-literatureYao QNational Natural Science Foundation of ChinaMinistry of Science and Technology of the People's Republic of China102765https://www.ebi.ac.uk/biostudies/studies/S-EPMC9808002biostudies-literatureUnknown299(1)Hyperlipidemia characterized by high blood levels of free fatty acids (FFAs) is important for the progression of inflammatory cardiovascular diseases. Integrin β₁ is a transmembrane receptor that drives various cellular functions, including differentiation, migration, and phagocytosis. However, the underlying mechanisms modifying integrin β₁ protein and activity in mediating monocyte/macrophage adhesion to endothelium remain poorly understood. In this study, we demonstrated that integrin β₁ protein underwent S-nitrosylation in response to nitrosative stress in macrophages. To examine the effect of elevated levels of FFA on the modulation of integrin β₁ expression, we treated the macrophages with a combination of oleic acid and palmitic acid (2:1)The Journal of biological chemistryFree fatty acids stabilize integrin β₁via S-nitrosylation to promote monocyte-endothelial adhesion.PMC98080028177049791939108816003892018YFA08006008207082981830015Wang NLiu JXiao LXie XZhao WDu XLai BYao QJiang TWang HZhao ZCui QfalseFree fatty acids stabilize integrin β₁via S-nitrosylation to promote monocyte-endothelial adhesion.Hyperlipidemia characterized by high blood levels of free fatty acids (FFAs) is important for the progression of inflammatory cardiovascular diseases. Integrin β₁ is a transmembrane receptor that drives various cellular functions, including differentiation, migration, and phagocytosis. However, the underlying mechanisms modifying integrin β₁ protein and activity in mediating monocyte/macrophage adhesion to endothelium remain poorly understood. In this study, we demonstrated that integrin β₁ protein underwent S-nitrosylation in response to nitrosative stress in macrophages. To examine the effect of elevated levels of FFA on the modulation of integrin β₁ expression, we treated the macrophages with a combination of oleic acid and palmitic acid (2:1)2022-01-01T00:00:00Z2022 Dec2025-04-04T21:04:41.119Z2025-04-04T21:04:41.119ZS-EPMC98080023647042310.1016/j.jbc.2022.102765