biostudies-literatureUnknown13Yang YBenzylisoquinoline alkaloids (BIAs) are a large family of plant natural products with important pharmaceutical applications. <i>Sinomenium acutum</i> is a medicinal plant from the Menispermaceae family and has been used to treat rheumatoid arthritis for hundreds of years. <i>Sinomenium acutum</i> contains more than 50 BIAs, and sinomenine is a representative BIA from this plant. Sinomenine was found to have preventive and curative effects on opioid dependence. Despite the broad applications of <i>S. acutum</i>, investigation on the biosynthetic pathways of BIAs from <i>S. acutum</i> is limited. In this study, we comprehensively analyzed the transcriptome data and BIAs in the root, stem, leaf, and seed of <i>S. acutum</i>. Metabolic analysis showed a noticeable difference in BIA contents inFrontiers in plant science1086335https://www.ebi.ac.uk/biostudies/studies/S-EPMC9808091biostudies-literatureFull-length transcriptome and metabolite analysis reveal reticuline epimerase-independent pathways for benzylisoquinoline alkaloids biosynthesis in <i>Sinomenium acutum</i>.PMC9808091Yang YSun RWang CHuang XXue LYin MSun YWang ZYan XfalseFull-length transcriptome and metabolite analysis reveal reticuline epimerase-independent pathways for benzylisoquinoline alkaloids biosynthesis in <i>Sinomenium acutum</i>.Benzylisoquinoline alkaloids (BIAs) are a large family of plant natural products with important pharmaceutical applications. <i>Sinomenium acutum</i> is a medicinal plant from the Menispermaceae family and has been used to treat rheumatoid arthritis for hundreds of years. <i>Sinomenium acutum</i> contains more than 50 BIAs, and sinomenine is a representative BIA from this plant. Sinomenine was found to have preventive and curative effects on opioid dependence. Despite the broad applications of <i>S. acutum</i>, investigation on the biosynthetic pathways of BIAs from <i>S. acutum</i> is limited. In this study, we comprehensively analyzed the transcriptome data and BIAs in the root, stem, leaf, and seed of <i>S. acutum</i>. Metabolic analysis showed a noticeable difference in BIA contents in2022-01-01T00:00:00Z20222025-04-04T13:52:52.778Z2025-04-04T13:52:52.778ZS-EPMC98080913660596810.3389/fpls.2022.1086335