{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Evans L"],"funding":["Swiss National Science Foundation","Biotechnology and Biological Sciences Research Council","Engineering and Physical Sciences Research Council"],"pagination":["94-100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9811510"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(1)"],"pubmed_abstract":["A number of Gram-negative bacteria such as <i>Pseudomonas aeruginosa</i> are becoming resistant to front-line antibiotics. Consequently, there is a pressing need to find alternative bio-molecular targets for the development of new drugs. Since non-canonical DNA structures such as guanine-quadruplexes (G4s) have been implicated in regulating transcription, we were interested in determining whether there are putative quadruplex-forming sequences (PQS) in the genome of <i>Pseudomonas aeruginosa</i>. Using bioinformatic tools, we screened 36 genes potentially relevant to drug resistance for the presence of PQS and 10 of these were selected for biophysical characterisation (<i>i.e.</i> circular dichroism and thermal difference UV/Vis spectroscopy). These studies showed that three of these G-ric"],"journal":["RSC chemical biology"],"pubmed_title":["Identification and characterisation of G-quadruplex DNA-forming sequences in the <i>Pseudomonas aeruginosa</i> genome."],"pmcid":["PMC9811510"],"funding_grant_id":["BB/R00174X/1","P2LAP3-148450","BB/L007959/1","148450"],"pubmed_authors":["Filloux A","Jamshidi S","Evans L","Vilar R","Plavec J","Rahman KM","Kotar A","Valentini M"],"additional_accession":[]},"is_claimable":false,"name":"Identification and characterisation of G-quadruplex DNA-forming sequences in the <i>Pseudomonas aeruginosa</i> genome.","description":"A number of Gram-negative bacteria such as <i>Pseudomonas aeruginosa</i> are becoming resistant to front-line antibiotics. Consequently, there is a pressing need to find alternative bio-molecular targets for the development of new drugs. Since non-canonical DNA structures such as guanine-quadruplexes (G4s) have been implicated in regulating transcription, we were interested in determining whether there are putative quadruplex-forming sequences (PQS) in the genome of <i>Pseudomonas aeruginosa</i>. Using bioinformatic tools, we screened 36 genes potentially relevant to drug resistance for the presence of PQS and 10 of these were selected for biophysical characterisation (<i>i.e.</i> circular dichroism and thermal difference UV/Vis spectroscopy). These studies showed that three of these G-ric","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-03-27T16:49:04.202Z","creation":"2025-04-04T14:27:05.906Z"},"accession":"S-EPMC9811510","cross_references":{"pubmed":["36685252"],"doi":["10.1039/d2cb00205a"]}}