biostudies-literatureEvans LSwiss National Science FoundationBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Council94-100https://www.ebi.ac.uk/biostudies/studies/S-EPMC9811510biostudies-literatureUnknown4(1)A number of Gram-negative bacteria such as <i>Pseudomonas aeruginosa</i> are becoming resistant to front-line antibiotics. Consequently, there is a pressing need to find alternative bio-molecular targets for the development of new drugs. Since non-canonical DNA structures such as guanine-quadruplexes (G4s) have been implicated in regulating transcription, we were interested in determining whether there are putative quadruplex-forming sequences (PQS) in the genome of <i>Pseudomonas aeruginosa</i>. Using bioinformatic tools, we screened 36 genes potentially relevant to drug resistance for the presence of PQS and 10 of these were selected for biophysical characterisation (<i>i.e.</i> circular dichroism and thermal difference UV/Vis spectroscopy). These studies showed that three of these G-ricRSC chemical biologyIdentification and characterisation of G-quadruplex DNA-forming sequences in the <i>Pseudomonas aeruginosa</i> genome.PMC9811510BB/R00174X/1P2LAP3-148450BB/L007959/1148450Filloux AJamshidi SEvans LVilar RPlavec JRahman KMKotar AValentini MfalseIdentification and characterisation of G-quadruplex DNA-forming sequences in the <i>Pseudomonas aeruginosa</i> genome.A number of Gram-negative bacteria such as <i>Pseudomonas aeruginosa</i> are becoming resistant to front-line antibiotics. Consequently, there is a pressing need to find alternative bio-molecular targets for the development of new drugs. Since non-canonical DNA structures such as guanine-quadruplexes (G4s) have been implicated in regulating transcription, we were interested in determining whether there are putative quadruplex-forming sequences (PQS) in the genome of <i>Pseudomonas aeruginosa</i>. Using bioinformatic tools, we screened 36 genes potentially relevant to drug resistance for the presence of PQS and 10 of these were selected for biophysical characterisation (<i>i.e.</i> circular dichroism and thermal difference UV/Vis spectroscopy). These studies showed that three of these G-ric2023-01-01T00:00:00Z2023 Jan2026-03-27T16:49:04.202Z2025-04-04T14:27:05.906ZS-EPMC98115103668525210.1039/d2cb00205a