biostudies-literatureTabula Sapiens Consortium*NIDDK NIH HHSNHLBI NIH HHSNIDCD NIH HHSeabl4896https://www.ebi.ac.uk/biostudies/studies/S-EPMC9812260biostudies-literatureUnknown376(6594)Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discoveScience (New York, N.Y.)The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans.PMC9812260P30 DK026743R01 DK126482R01 DC016892R01 DK128932T32 HL120824Travaglini KJHoushdaran SLoeb GBAaron KAKershner AMBean GRHemenez MWyss-Coray TVo KCChang CAXing GHuang FWNeuhofer PKumar MEJuanico JVMetzger RJGranados AAAfik SBeachy PAXu CChan CKFSilva OToland AHang YCalcuttawala KMekonen HMcGeever ARaquer-McKay HSeddighzadeh BAlcantara-Hernandez MMukherjee SSwarup AByrne ASonnenburg JLConcepcion WIrwin JCWu SMTabula Sapiens Consortium*Rando TAHarper WBotvinnik OBD'Amato GVanheusden RKelly BWu TTHJang SQi ZVan Treuren WTarashansky AJOlivieri JEWeinstein HNWClarke MFLi BSubramaniam VRPhansalkar RCulver RNMantri SCrasta SChang Sde Morree ARavikumar NGiudice LCGardner JMIdoyaga JSanagavarapu BAKim SCharu VGayoso ASpallino EWinkler JHuang XQuake SRSit RVEzran CRed-Horse KDehghannasiri RKong WSalehi ARao PVemuri VNPTan SYWang BPisco AOHo PYLeylek RKrasnow MACvijovic IWang PDethlefsen LWang SXie JWang WHuang KCPonnusamy RKim SKWang ZKuo AHSegal JTan MSpencer SPMuser TChen MPark MGoodyer WRAwayan KVorperian SKLi QSen SMorri MVeizades SSinha RTan WWu AJones RCMcAlpine PLSikandar SMartin BAGaldos FXSchaum NMahmoudabadi GNguyen TDCarter MMMarkovic MScott BColville ALiu SMaltzman JSBurhan DSwift MLu WJDetweiler AMOzawa MGRelman DAMrouj KStanley GSteffes LCYung ARKuo CSGillich ATrimm EPerez KReiter JYan JForgo EKolluru SAntony JNeff NFNguyen PSong HNeidlinger NBrown PAlmanzar NYosef NVijayakumar SZhang YSalzman JBeyersdorf BKiss BSasagawa KHayashi AKarkanias JPuluca NBulthaup BfalseThe Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans.Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discove2022-01-01T00:00:00Z2022 May2026-03-17T15:48:52.91Z2025-04-07T05:06:31.339ZS-EPMC98122603554940410.1126/science.abl4896