{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Banahene N"],"funding":["Camille and Henry Dreyfus Foundation","NIAID NIH HHS","National Institutes of Health","NIGMS NIH HHS","National Science Foundation"],"pagination":["e202213563"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9812908"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["62(2)"],"pubmed_abstract":["Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool."],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing."],"pmcid":["PMC9812908"],"funding_grant_id":["AI138238","R35 GM136365","2117338","1654408","DP2 AI138238","TH-17-034","GM136365"],"pubmed_authors":["Biegas KJ","Van Nieuwenhze MS","Banahene N","Gepford DM","Lepori I","Swanson DH","Obregon-Henao A","Hsu YP","Taylor ZE","Murphy BA","Siegrist MS","Swarts BM"],"additional_accession":[]},"is_claimable":false,"name":"A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing.","description":"Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-03T21:31:04.485Z","creation":"2025-04-03T21:31:04.485Z"},"accession":"S-EPMC9812908","cross_references":{"pubmed":["36346622"],"doi":["10.1002/anie.202213563"]}}