{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Delafield DG"],"funding":["Division of Chemistry","National Center for Research Resources","Wisconsin Alumni Research Foundation","Directorate for Biological Sciences","National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","NIA NIH HHS","NCRR NIH HHS","NIH Office of the Director","University of Wisconsin-Madison","NIH HHS","National Institute on Aging"],"pagination":["64-74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9812930"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["34(1)"],"pubmed_abstract":["Mass spectrometry-based discovery glycoproteomics is highly dependent on the use of chromatography paradigms amenable to analyte retention and separation. When compared against established stationary phases such as reversed-phase and hydrophilic interaction liquid chromatography, reports utilizing porous graphitic carbon have detailed its numerous advantages. Recent efforts have highlighted the utility in porous graphitic carbon in high-throughput glycoproteomics, principally through enhanced profiling depth and liquid-phase resolution at higher column temperatures. However, increasing column temperature has been shown to impart disparaging effects in glycopeptide identification. Herein we further elucidate this trend, describing qualitative and semiquantitative effects of increased column"],"journal":["Journal of the American Society for Mass Spectrometry"],"pubmed_title":["Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes."],"pmcid":["PMC9812930"],"funding_grant_id":["S10RR029531","S10 OD028473","S10 OD025084","R01 DK127081","R01 DK071801","R21 AG065728","RF1 AG052324","S10OD028473","S10OD025084","IOS-2010789","R21AG065728","U54 DK104310","R01 DK131175","U54DK104310","S10 RR029531","2108223"],"pubmed_authors":["Miles HN","Ricke WA","Delafield DG","Li L"],"additional_accession":[]},"is_claimable":false,"name":"Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes.","description":"Mass spectrometry-based discovery glycoproteomics is highly dependent on the use of chromatography paradigms amenable to analyte retention and separation. When compared against established stationary phases such as reversed-phase and hydrophilic interaction liquid chromatography, reports utilizing porous graphitic carbon have detailed its numerous advantages. Recent efforts have highlighted the utility in porous graphitic carbon in high-throughput glycoproteomics, principally through enhanced profiling depth and liquid-phase resolution at higher column temperatures. However, increasing column temperature has been shown to impart disparaging effects in glycopeptide identification. Herein we further elucidate this trend, describing qualitative and semiquantitative effects of increased column","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-28T03:47:18.916Z","creation":"2025-04-06T00:53:16.96Z"},"accession":"S-EPMC9812930","cross_references":{"pubmed":["36450095"],"doi":["10.1021/jasms.2c00249"]}}