biostudies-literatureUnknown13Frentzel SDeutsche ForschungsgemeinschaftIκB proteins regulate the inhibition and activation of NF-κB transcription factor complexes. While classical IκB proteins keep NF-κB complexes inactive in the cytoplasm, atypical IκB proteins act on activated NF-κB complexes located in the nucleus. Most of the knowledge regarding the function of IκB proteins has been collected <i>in vitro</i>, while far less is known regarding their impact on activation and regulation of immune responses during <i>in vivo</i> infections. Combining <i>in vivo Listeria monocytogenes</i> (Lm) infection with comparative <i>ex vivo</i> transcriptional profiling of the hepatic response to the pathogen we observed that in contrast to wild type mice that mounted a robust inflammatory response, IκB_NS-deficiency was generally associated with a transcriptiFrontiers in immunology1028789https://www.ebi.ac.uk/biostudies/studies/S-EPMC9813228biostudies-literatureIκB_NS-deficiency protects mice from fatal <i>Listeria monocytogenes</i> infection by blunting pro-inflammatory signature in Ly6C^high monocytes and preventing exaggerated innate immune responses.PMC9813228Jeron APausder AVolckmar JSchmitz IBruder DKershaw OFrentzel SfalseIκB_NS-deficiency protects mice from fatal <i>Listeria monocytogenes</i> infection by blunting pro-inflammatory signature in Ly6C^high monocytes and preventing exaggerated innate immune responses.IκB proteins regulate the inhibition and activation of NF-κB transcription factor complexes. While classical IκB proteins keep NF-κB complexes inactive in the cytoplasm, atypical IκB proteins act on activated NF-κB complexes located in the nucleus. Most of the knowledge regarding the function of IκB proteins has been collected <i>in vitro</i>, while far less is known regarding their impact on activation and regulation of immune responses during <i>in vivo</i> infections. Combining <i>in vivo Listeria monocytogenes</i> (Lm) infection with comparative <i>ex vivo</i> transcriptional profiling of the hepatic response to the pathogen we observed that in contrast to wild type mice that mounted a robust inflammatory response, IκB_NS-deficiency was generally associated with a transcripti2022-01-01T00:00:00Z20222026-04-07T17:33:19.802Z2025-04-06T00:52:50.606ZS-EPMC98132283661834410.3389/fimmu.2022.1028789