biostudies-literatureUnknown9Weng CH<h4>Background</h4>Endometrial hyperplasia (EH), particularly with atypia, is considered an antecedent of endometrial adenocarcinoma. In this study, we aimed to apply massively parallel sequencing of endometrial lavage specimens for the detection of cancer-associated mutations in atypical (AEH) and non-atypical endometrial hyperplasia (NEH). The identified alterations were compared with those detected in tissue samples.<h4>Materials and methods</h4>Endometrial lavage specimens and parallel biopsy samples (<i>n</i> = 11 for AEH and <i>n</i> = 9 for NEH) were obtained from 18 women (9 with AEH and 9 with NEH) who received an office hysteroscopy for suspected endometrial lesions. All samples were tested for somatic mutations in hotspot regions of 72 cancer-associated genes by massively parallFrontiers in medicine1090788https://www.ebi.ac.uk/biostudies/studies/S-EPMC9813484biostudies-literatureMassively parallel sequencing of endometrial lavage specimens for the detection of cancer-associated mutations in atypical and non-atypical endometrial hyperplasia.PMC9813484Huang HJWang CJLai CHRo AWeng CHChao ASWu RCTsai CLWu KYLin CYChao AfalseMassively parallel sequencing of endometrial lavage specimens for the detection of cancer-associated mutations in atypical and non-atypical endometrial hyperplasia.<h4>Background</h4>Endometrial hyperplasia (EH), particularly with atypia, is considered an antecedent of endometrial adenocarcinoma. In this study, we aimed to apply massively parallel sequencing of endometrial lavage specimens for the detection of cancer-associated mutations in atypical (AEH) and non-atypical endometrial hyperplasia (NEH). The identified alterations were compared with those detected in tissue samples.<h4>Materials and methods</h4>Endometrial lavage specimens and parallel biopsy samples (<i>n</i> = 11 for AEH and <i>n</i> = 9 for NEH) were obtained from 18 women (9 with AEH and 9 with NEH) who received an office hysteroscopy for suspected endometrial lesions. All samples were tested for somatic mutations in hotspot regions of 72 cancer-associated genes by massively parall2022-01-01T00:00:00Z20222025-04-04T12:37:23.637Z2025-04-04T12:37:23.637ZS-EPMC98134843661962010.3389/fmed.2022.1090788