{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cai J"],"funding":["Intramural NIH HHS","National Natural Science Foundation of China","National Cancer Institute"],"pagination":["e21496"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9813681"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(5)"],"pubmed_abstract":["Saururus chinensis (S chinensis) has been used as an herb to treat edema, jaundice, and gonorrhea. Manassantin B (MNSB), a dineolignan isolated from S chinensis, was identified as a potent adipogenesis/lipogenesis inhibitor (IC<sub>50</sub>  = 9.3 nM). To explore the underlying mechanism, both adipogenesis and lipogenesis were measured in differentiated 3T3-L1 preadipocytes, murine primary preadipocytes and adipose tissue explants upon MNSB treatment. Key regulators of adipogenesis/lipogenesis were downregulated by MNSB treatment, mainly resulting from increased phosphorylation of AMPK which was identified as a vital regulator of adipogenesis and lipogenesis. Moreover, MNSB did not increase AMPK phosphorylation in 3T3-L1 cells transfected with Prkaa1 (encoding protein kinase AMP-activated "],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["Manassantin B attenuates obesity by inhibiting adipogenesis and lipogenesis in an AMPK dependent manner."],"pmcid":["PMC9813681"],"funding_grant_id":["81573310","ZIA BC005708"],"pubmed_authors":["Sun L","Yuan S","Qiong G","Li C","Cai J","Luo Y","Gonzalez FJ","Xu J"],"additional_accession":[]},"is_claimable":false,"name":"Manassantin B attenuates obesity by inhibiting adipogenesis and lipogenesis in an AMPK dependent manner.","description":"Saururus chinensis (S chinensis) has been used as an herb to treat edema, jaundice, and gonorrhea. Manassantin B (MNSB), a dineolignan isolated from S chinensis, was identified as a potent adipogenesis/lipogenesis inhibitor (IC<sub>50</sub>  = 9.3 nM). To explore the underlying mechanism, both adipogenesis and lipogenesis were measured in differentiated 3T3-L1 preadipocytes, murine primary preadipocytes and adipose tissue explants upon MNSB treatment. Key regulators of adipogenesis/lipogenesis were downregulated by MNSB treatment, mainly resulting from increased phosphorylation of AMPK which was identified as a vital regulator of adipogenesis and lipogenesis. Moreover, MNSB did not increase AMPK phosphorylation in 3T3-L1 cells transfected with Prkaa1 (encoding protein kinase AMP-activated ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2025-04-04T13:52:56.822Z","creation":"2025-04-04T13:52:56.822Z"},"accession":"S-EPMC9813681","cross_references":{"pubmed":["33904622"],"doi":["10.1096/fj.202002126RR","10.1096/fj.202002126rr"]}}