biostudies-literatureCai JIntramural NIH HHSNational Natural Science Foundation of ChinaNational Cancer Institutee21496https://www.ebi.ac.uk/biostudies/studies/S-EPMC9813681biostudies-literatureUnknown35(5)Saururus chinensis (S chinensis) has been used as an herb to treat edema, jaundice, and gonorrhea. Manassantin B (MNSB), a dineolignan isolated from S chinensis, was identified as a potent adipogenesis/lipogenesis inhibitor (IC₅₀  = 9.3 nM). To explore the underlying mechanism, both adipogenesis and lipogenesis were measured in differentiated 3T3-L1 preadipocytes, murine primary preadipocytes and adipose tissue explants upon MNSB treatment. Key regulators of adipogenesis/lipogenesis were downregulated by MNSB treatment, mainly resulting from increased phosphorylation of AMPK which was identified as a vital regulator of adipogenesis and lipogenesis. Moreover, MNSB did not increase AMPK phosphorylation in 3T3-L1 cells transfected with Prkaa1 (encoding protein kinase AMP-activated FASEB journal : official publication of the Federation of American Societies for Experimental BiologyManassantin B attenuates obesity by inhibiting adipogenesis and lipogenesis in an AMPK dependent manner.PMC981368181573310ZIA BC005708Sun LYuan SQiong GLi CCai JLuo YGonzalez FJXu JfalseManassantin B attenuates obesity by inhibiting adipogenesis and lipogenesis in an AMPK dependent manner.Saururus chinensis (S chinensis) has been used as an herb to treat edema, jaundice, and gonorrhea. Manassantin B (MNSB), a dineolignan isolated from S chinensis, was identified as a potent adipogenesis/lipogenesis inhibitor (IC₅₀  = 9.3 nM). To explore the underlying mechanism, both adipogenesis and lipogenesis were measured in differentiated 3T3-L1 preadipocytes, murine primary preadipocytes and adipose tissue explants upon MNSB treatment. Key regulators of adipogenesis/lipogenesis were downregulated by MNSB treatment, mainly resulting from increased phosphorylation of AMPK which was identified as a vital regulator of adipogenesis and lipogenesis. Moreover, MNSB did not increase AMPK phosphorylation in 3T3-L1 cells transfected with Prkaa1 (encoding protein kinase AMP-activated 2021-01-01T00:00:00Z2021 May2025-04-04T13:52:56.822Z2025-04-04T13:52:56.822ZS-EPMC98136813390462210.1096/fj.202002126RR10.1096/fj.202002126rr