{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Cai J"],"pubmed_abstract":["Wilt disease caused by <i>Phytophthora melonis</i> infection is one of the most serious threats to <i>Benincasa hispida</i> production. However, the mechanism of the response of <i>B. hispida</i> to a <i>P. melonis</i> infection remains largely unknown. In the present study, two <i>B. hispida</i> cultivars with different degrees of resistance to <i>P. melonis</i> were identified: B488 (a moderately resistant cultivar) and B214 (a moderately susceptible cultivar). RNA-seq was performed on <i>P. melonis</i>-infected B488 and B214 12 hours post infection (hpi). Compared with the control, 680 and 988 DEGs were respectively detected in B488 and B214. A KEGG pathway analysis combined with a cluster analysis revealed that phenylpropanoid biosynthesis, plant-pathogen interaction, the MAPK signaling pathway-plant, and plant hormone signal transduction were the most relevant pathways during the response of both B488 and B214 to <i>P. melonis</i> infection, as well as the differentially expressed genes in the two cultivars. In addition, a cluster analysis of transcription factor genes in DEGs identified four genes upregulated in B488 but not in B214 at 6 hpi and 12 hpi, which was confirmed by qRT-PCR. These were candidate genes for elucidating the mechanism of the <i>B. hispida</i> response to <i>P. melonis</i> infection and laying the foundation for the improvement of <i>B. hispida</i>."],"journal":["Frontiers in plant science"],"pagination":["1106123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9815465"],"repository":["biostudies-literature"],"pubmed_title":["A transcriptome analysis of <i>Benincasa hispida</i> revealed the pathways and genes involved in response to <i>Phytophthora melonis</i> infection."],"pmcid":["PMC9815465"],"pubmed_authors":["Xie D","Yan J","Cai J","Jiang B","Yang S","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"A transcriptome analysis of <i>Benincasa hispida</i> revealed the pathways and genes involved in response to <i>Phytophthora melonis</i> infection.","description":"Wilt disease caused by <i>Phytophthora melonis</i> infection is one of the most serious threats to <i>Benincasa hispida</i> production. However, the mechanism of the response of <i>B. hispida</i> to a <i>P. melonis</i> infection remains largely unknown. In the present study, two <i>B. hispida</i> cultivars with different degrees of resistance to <i>P. melonis</i> were identified: B488 (a moderately resistant cultivar) and B214 (a moderately susceptible cultivar). RNA-seq was performed on <i>P. melonis</i>-infected B488 and B214 12 hours post infection (hpi). Compared with the control, 680 and 988 DEGs were respectively detected in B488 and B214. A KEGG pathway analysis combined with a cluster analysis revealed that phenylpropanoid biosynthesis, plant-pathogen interaction, the MAPK signaling pathway-plant, and plant hormone signal transduction were the most relevant pathways during the response of both B488 and B214 to <i>P. melonis</i> infection, as well as the differentially expressed genes in the two cultivars. In addition, a cluster analysis of transcription factor genes in DEGs identified four genes upregulated in B488 but not in B214 at 6 hpi and 12 hpi, which was confirmed by qRT-PCR. These were candidate genes for elucidating the mechanism of the <i>B. hispida</i> response to <i>P. melonis</i> infection and laying the foundation for the improvement of <i>B. hispida</i>.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2024-11-12T18:01:54.857Z","creation":"2024-11-12T18:01:54.857Z"},"accession":"S-EPMC9815465","cross_references":{"pubmed":["36618646"],"doi":["10.3389/fpls.2022.1106123"]}}