biostudies-literatureLi QNational Natural Science Foundation of ChinaPostgraduate research & practice innovation program of Jiangsu provinceQinglan Project of Jiangsu Province of Chinae13336https://www.ebi.ac.uk/biostudies/studies/S-EPMC9816938biostudies-literatureUnknown56(1)<h4>Objectives</h4>Transforming growth factor β1 (TGF-β1), an essential cytokine belongs to TGF-β superfamily, is crucial for female fertility. Increasing evidence show that long noncoding RNAs (lncRNAs) influence the state of granulosa cells (GCs). This study aimed to detect the effects of TGF-β1 on the lncRNA transcriptome, and investigate whether lncRNAs mediate the functions of TGF-β1 in GCs.<h4>Material and methods</h4>RNA-seq and bioinformatics analyses were performed to identify and characterize the differentially expressed lncRNAs (DElncRNAs). The regulatory mechanism of TGF-β1 to lncRNA transcriptome was analyzed by chromatin immunoprecipitation. The effects of lncRNAs on the antiapoptotic and proproliferative functions of TGF-β1 were examined by morphological analysis, fluorescenCell proliferationTGF-β1 regulates the lncRNA transcriptome of ovarian granulosa cells in a transcription activity-dependent manner.PMC9816938KYCX19-0507202031902130Yang LHuo YWang SDu XLi QfalseTGF-β1 regulates the lncRNA transcriptome of ovarian granulosa cells in a transcription activity-dependent manner.<h4>Objectives</h4>Transforming growth factor β1 (TGF-β1), an essential cytokine belongs to TGF-β superfamily, is crucial for female fertility. Increasing evidence show that long noncoding RNAs (lncRNAs) influence the state of granulosa cells (GCs). This study aimed to detect the effects of TGF-β1 on the lncRNA transcriptome, and investigate whether lncRNAs mediate the functions of TGF-β1 in GCs.<h4>Material and methods</h4>RNA-seq and bioinformatics analyses were performed to identify and characterize the differentially expressed lncRNAs (DElncRNAs). The regulatory mechanism of TGF-β1 to lncRNA transcriptome was analyzed by chromatin immunoprecipitation. The effects of lncRNAs on the antiapoptotic and proproliferative functions of TGF-β1 were examined by morphological analysis, fluorescen2023-01-01T00:00:00Z2023 Jan2025-04-21T16:17:18.626Z2025-04-21T16:17:18.626ZS-EPMC98169383612509510.1111/cpr.13336