biostudies-literatureUnknown13(1)Cheng MCLymphocystic disease affects over 150 species of marine and freshwater fish worldwide. In this study, the lymphocystis pathogen was found in 2 (<i>Amphiprion ocellaris</i> and <i>Amphiprion clarkii</i>) of the 9 species of clownfish. Detection of lymphocystis disease virus (LCDV) was based on histopathological study, electron microscope observation of virus particles and gene sequence analysis from the MCP region. Infected <i>A. ocellaris</i> hosts showed sparse, multifocal, white, stiff, papilloma-like nodules on the body, skin, gills and fins; while, on <i>A. clarkia</i>, nodules were found on the operculum skin. Histopathologic study showed lymphocystic cells with an irregular nucleus, enlarged cytoplasm and intracytoplasmic inclusion bodies surrounded by the cell membrane. The viral paAnimals : an open access journal from MDPI153https://www.ebi.ac.uk/biostudies/studies/S-EPMC9817495biostudies-literatureLymphocystis Disease Virus Infection in Clownfish <i>Amphiprion ocellaris</i> and <i>Amphiprion clarkii</i> in Taiwan.PMC9817495Cheng MCWang PCTsai MAKuo YTChen SCHo YSSee MSfalseLymphocystis Disease Virus Infection in Clownfish <i>Amphiprion ocellaris</i> and <i>Amphiprion clarkii</i> in Taiwan.Lymphocystic disease affects over 150 species of marine and freshwater fish worldwide. In this study, the lymphocystis pathogen was found in 2 (<i>Amphiprion ocellaris</i> and <i>Amphiprion clarkii</i>) of the 9 species of clownfish. Detection of lymphocystis disease virus (LCDV) was based on histopathological study, electron microscope observation of virus particles and gene sequence analysis from the MCP region. Infected <i>A. ocellaris</i> hosts showed sparse, multifocal, white, stiff, papilloma-like nodules on the body, skin, gills and fins; while, on <i>A. clarkia</i>, nodules were found on the operculum skin. Histopathologic study showed lymphocystic cells with an irregular nucleus, enlarged cytoplasm and intracytoplasmic inclusion bodies surrounded by the cell membrane. The viral pa2022-01-01T00:00:00Z2022 Dec2025-04-05T11:24:05.209Z2025-04-05T11:24:05.209ZS-EPMC98174953661176210.3390/ani13010153