biostudies-literatureTai JScience and Technology Department of Zhejiang Province52https://www.ebi.ac.uk/biostudies/studies/S-EPMC9818276biostudies-literatureUnknown12(1)This study was aimed to investigate the structural and immunological properties of parvalbumin from mandarin fish during the Maillard reaction. The microwave-assisted the Maillard reaction was optimized by orthogonal designed experiments. The results showed that the type of sugar and heating time had a significant effect on the Maillard reaction (p < 0.05). The SDS-PAGE analysis displayed that the molecular weight of parvalbumin in mandarin fish changed after being glycated with the Maillard reaction. The glycated parvalbumin was analyzed by Nano-LC-MS/MS and eleven glycation sites as well as five glycation groups were identified. By using the indirect competitive ELISA method, it was found that microwave heating gave a higher desensitization ability of mandarin fish parvalbumin than inducFoods (Basel, Switzerland)Structural Property, Immunoreactivity and Gastric Digestion Characteristics of Glycated Parvalbumin from Mandarin Fish (<i>Siniperca chuaisi</i>) during Microwave-Assisted Maillard Reaction.PMC9818276LGN18C200016Li WZhang FLiang XLu YQiao DHuang XHu HZhang HTai JfalseStructural Property, Immunoreactivity and Gastric Digestion Characteristics of Glycated Parvalbumin from Mandarin Fish (<i>Siniperca chuaisi</i>) during Microwave-Assisted Maillard Reaction.This study was aimed to investigate the structural and immunological properties of parvalbumin from mandarin fish during the Maillard reaction. The microwave-assisted the Maillard reaction was optimized by orthogonal designed experiments. The results showed that the type of sugar and heating time had a significant effect on the Maillard reaction (p < 0.05). The SDS-PAGE analysis displayed that the molecular weight of parvalbumin in mandarin fish changed after being glycated with the Maillard reaction. The glycated parvalbumin was analyzed by Nano-LC-MS/MS and eleven glycation sites as well as five glycation groups were identified. By using the indirect competitive ELISA method, it was found that microwave heating gave a higher desensitization ability of mandarin fish parvalbumin than induc2022-01-01T00:00:00Z2022 Dec2025-05-29T16:22:39.54Z2025-04-04T20:33:08.212ZS-EPMC98182763661326810.3390/foods12010052