{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jia Y"],"funding":["China Scholarship Council","ARC","Centre Franco-Indian pour la Promotion de la recherche Avancée","Ligue Régionale contre le Cancer","Fondation pour la Recherche Médicale"],"pagination":["200"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9818449"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Biological pathways rely on the formation of intricate protein interaction networks called interactomes. Getting a comprehensive map of interactomes implies the development of tools that allow one to capture transient and low-affinity protein-protein interactions (PPIs) in live conditions. Here we presented an experimental strategy: the Cell-PCA (cell-based protein complementation assay), which was based on bimolecular fluorescence complementation (BiFC) for ORFeome-wide screening of proteins that interact with different bait proteins in the same live cell context, by combining high-throughput sequencing method. The specificity and sensitivity of the Cell-PCA was established by using a wild-type and a single-amino-acid-mutated HOXA9 protein, and the approach was subsequently applied to sev"],"journal":["Cells"],"pubmed_title":["A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes."],"pmcid":["PMC9818449"],"funding_grant_id":["5503-P","PJA20191209567","201708070003","160896","119030"],"pubmed_authors":["Merabet S","Kundlacz C","Vanacker JM","Reboulet J","Hughes S","Hajj Sleiman N","Jia Y","Tribollet V","Gillet B","Forcet C","Bleicher F"],"additional_accession":[]},"is_claimable":false,"name":"A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX Interactomes.","description":"Biological pathways rely on the formation of intricate protein interaction networks called interactomes. Getting a comprehensive map of interactomes implies the development of tools that allow one to capture transient and low-affinity protein-protein interactions (PPIs) in live conditions. Here we presented an experimental strategy: the Cell-PCA (cell-based protein complementation assay), which was based on bimolecular fluorescence complementation (BiFC) for ORFeome-wide screening of proteins that interact with different bait proteins in the same live cell context, by combining high-throughput sequencing method. The specificity and sensitivity of the Cell-PCA was established by using a wild-type and a single-amino-acid-mutated HOXA9 protein, and the approach was subsequently applied to sev","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-26T06:03:11.726Z","creation":"2025-04-06T11:43:38.491Z"},"accession":"S-EPMC9818449","cross_references":{"pubmed":["36611993"],"doi":["10.3390/cells12010200"]}}