{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Canella R"],"funding":["F. Brugnoli by FIRD2022","N. Bianchi by FAR2021","V. Bertagnolo by FAR2021","N. Bianchi by FIR2021","R. Canella by FAR2020","S. Volinia by FAR2021"],"pagination":["178"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9818547"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Since the multifunctionality of transglutaminase 2 (TG2) includes extra- and intracellular functions, we investigated the effects of intracellular administration of TG2 inhibitors in three breast cancer cell lines, MDA-MB-231, MDA-MB-436 and MDA-MB-468, which are representative of different triple-negative phenotypes, using a patch-clamp technique. The first cell line has a highly voltage-dependent a membrane current, which is low in the second and almost absent in the third one. While applying a voltage protocol to responsive single cells, injection of TG2 inhibitors triggered a significant decrease of the current in MDA-MB-231 that we attributed to voltage-dependent K<sup>+</sup> channels using the specific inhibitors 4-aminopyridine and astemizole. Since the Kv10.1 channel plays a domin"],"journal":["Cancers"],"pubmed_title":["A Multidisciplinary Approach Establishes a Link between Transglutaminase 2 and the Kv10.1 Voltage-Dependent K<sup>+</sup> Channel in Breast Cancer."],"pmcid":["PMC9818547"],"funding_grant_id":["FAR2191074","FIR2191318"],"pubmed_authors":["Bertagnolo V","Brugnoli F","Bergamini CM","Ferrari E","Gallo M","Keillor JW","Canella R","Terrazzan A","Bianchi N","Grassilli S","Gates EWJ","Volinia S"],"additional_accession":[]},"is_claimable":false,"name":"A Multidisciplinary Approach Establishes a Link between Transglutaminase 2 and the Kv10.1 Voltage-Dependent K<sup>+</sup> Channel in Breast Cancer.","description":"Since the multifunctionality of transglutaminase 2 (TG2) includes extra- and intracellular functions, we investigated the effects of intracellular administration of TG2 inhibitors in three breast cancer cell lines, MDA-MB-231, MDA-MB-436 and MDA-MB-468, which are representative of different triple-negative phenotypes, using a patch-clamp technique. The first cell line has a highly voltage-dependent a membrane current, which is low in the second and almost absent in the third one. While applying a voltage protocol to responsive single cells, injection of TG2 inhibitors triggered a significant decrease of the current in MDA-MB-231 that we attributed to voltage-dependent K<sup>+</sup> channels using the specific inhibitors 4-aminopyridine and astemizole. Since the Kv10.1 channel plays a domin","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T20:33:12.045Z","creation":"2024-11-08T22:05:56.703Z"},"accession":"S-EPMC9818547","cross_references":{"pubmed":["36612174"],"doi":["10.3390/cancers15010178"]}}