biostudies-literatureCanella RF. Brugnoli by FIRD2022N. Bianchi by FAR2021V. Bertagnolo by FAR2021N. Bianchi by FIR2021R. Canella by FAR2020S. Volinia by FAR2021178https://www.ebi.ac.uk/biostudies/studies/S-EPMC9818547biostudies-literatureUnknown15(1)Since the multifunctionality of transglutaminase 2 (TG2) includes extra- and intracellular functions, we investigated the effects of intracellular administration of TG2 inhibitors in three breast cancer cell lines, MDA-MB-231, MDA-MB-436 and MDA-MB-468, which are representative of different triple-negative phenotypes, using a patch-clamp technique. The first cell line has a highly voltage-dependent a membrane current, which is low in the second and almost absent in the third one. While applying a voltage protocol to responsive single cells, injection of TG2 inhibitors triggered a significant decrease of the current in MDA-MB-231 that we attributed to voltage-dependent K⁺ channels using the specific inhibitors 4-aminopyridine and astemizole. Since the Kv10.1 channel plays a dominCancersA Multidisciplinary Approach Establishes a Link between Transglutaminase 2 and the Kv10.1 Voltage-Dependent K⁺ Channel in Breast Cancer.PMC9818547FAR2191074FIR2191318Bertagnolo VBrugnoli FBergamini CMFerrari EGallo MKeillor JWCanella RTerrazzan ABianchi NGrassilli SGates EWJVolinia SfalseA Multidisciplinary Approach Establishes a Link between Transglutaminase 2 and the Kv10.1 Voltage-Dependent K⁺ Channel in Breast Cancer.Since the multifunctionality of transglutaminase 2 (TG2) includes extra- and intracellular functions, we investigated the effects of intracellular administration of TG2 inhibitors in three breast cancer cell lines, MDA-MB-231, MDA-MB-436 and MDA-MB-468, which are representative of different triple-negative phenotypes, using a patch-clamp technique. The first cell line has a highly voltage-dependent a membrane current, which is low in the second and almost absent in the third one. While applying a voltage protocol to responsive single cells, injection of TG2 inhibitors triggered a significant decrease of the current in MDA-MB-231 that we attributed to voltage-dependent K⁺ channels using the specific inhibitors 4-aminopyridine and astemizole. Since the Kv10.1 channel plays a domin2022-01-01T00:00:00Z2022 Dec2025-04-04T20:33:12.045Z2024-11-08T22:05:56.703ZS-EPMC98185473661217410.3390/cancers15010178