{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Costa TFR"],"funding":["Isaac Newton Trust","National Council for Scientific and Technological Development","UK Research and Innovation","Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro"],"pagination":["656"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9820468"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(1)"],"pubmed_abstract":["The protozoan <i>Trypanosoma brucei rhodesiense</i> causes Human African Trypanosomiasis, also known as sleeping sickness, and penetrates the central nervous system, leading to meningoencephalitis. The Cathepsin L-like cysteine peptidase of <i>T. b. rhodesiense</i> has been implicated in parasite penetration of the blood-brain barrier and its activity is modulated by the chagasin-family endogenous inhibitor of cysteine peptidases (ICP). To investigate the role of ICP in <i>T. b. rhodesiense</i> bloodstream form, <i>ICP</i>-null (Δ<i>icp</i>) mutants were generated, and lines re-expressing <i>ICP</i> (Δ<i>icp:ICP</i>). Lysates of Δ<i>icp</i> displayed increased E-64-sensitive cysteine peptidase activity and the mutant parasites traversed human brain microvascular endothelial cell (HBMEC) mo"],"journal":["International journal of molecular sciences"],"pubmed_title":["<i>Trypanosoma brucei rhodesiense</i> Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response."],"pmcid":["PMC9820468"],"funding_grant_id":["311208/2017-7","MR/N017269/1","MR/P027989/1","E26/202.655/2019"],"pubmed_authors":["Mottram JC","Costa TFR","Lima APCA","Goundry A","Morrot A","Grab DJ"],"additional_accession":[]},"is_claimable":false,"name":"<i>Trypanosoma brucei rhodesiense</i> Inhibitor of Cysteine Peptidase (ICP) Is Required for Virulence in Mice and to Attenuate the Inflammatory Response.","description":"The protozoan <i>Trypanosoma brucei rhodesiense</i> causes Human African Trypanosomiasis, also known as sleeping sickness, and penetrates the central nervous system, leading to meningoencephalitis. The Cathepsin L-like cysteine peptidase of <i>T. b. rhodesiense</i> has been implicated in parasite penetration of the blood-brain barrier and its activity is modulated by the chagasin-family endogenous inhibitor of cysteine peptidases (ICP). To investigate the role of ICP in <i>T. b. rhodesiense</i> bloodstream form, <i>ICP</i>-null (Δ<i>icp</i>) mutants were generated, and lines re-expressing <i>ICP</i> (Δ<i>icp:ICP</i>). Lysates of Δ<i>icp</i> displayed increased E-64-sensitive cysteine peptidase activity and the mutant parasites traversed human brain microvascular endothelial cell (HBMEC) mo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-21T16:21:27.446Z","creation":"2025-04-21T16:21:27.446Z"},"accession":"S-EPMC9820468","cross_references":{"pubmed":["36614101"],"doi":["10.3390/ijms24010656"]}}