biostudies-literatureChen Searmarked fund for CARSNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaKey Research and Development Program of Hubei Province673https://www.ebi.ac.uk/biostudies/studies/S-EPMC9820476biostudies-literatureUnknown24(1)The intestinal microbiota contributes to energy metabolism, but the molecular mechanisms involved remain less clear. Bacteria of the genus <i>Bacillus</i> regulate lipid metabolism in the host and are thus commonly used as beneficial probiotic supplements. In the present study, <i>Bacillus licheniformis</i> FA6 was selected to assess its role in modulating lipid metabolism of zebrafish (<i>Danio rerio</i>). Combining 16S rRNA high-throughput sequencing, micro-CT scan, metabolic parameters measurement, and gene expression analysis, we demonstrated that <i>B. licheniformis</i> FA6 changed the gut microbiota composition of zebrafish and increased both the Firmicutes/Bacteroidetes ratio and lipid accumulation. In terms of metabolites, <i>B. licheniformis</i> FA6 appeared to promote acetate proInternational journal of molecular sciences<i>Bacillus licheniformis</i> FA6 Affects Zebrafish Lipid Metabolism through Promoting Acetyl-CoA Synthesis and Inhibiting β-Oxidation.PMC98204762021BBA231CARS-45318726122020YFD0900303Zan ZZou HClements KDZhao WWu SYe WWang GChen Sfalse<i>Bacillus licheniformis</i> FA6 Affects Zebrafish Lipid Metabolism through Promoting Acetyl-CoA Synthesis and Inhibiting β-Oxidation.The intestinal microbiota contributes to energy metabolism, but the molecular mechanisms involved remain less clear. Bacteria of the genus <i>Bacillus</i> regulate lipid metabolism in the host and are thus commonly used as beneficial probiotic supplements. In the present study, <i>Bacillus licheniformis</i> FA6 was selected to assess its role in modulating lipid metabolism of zebrafish (<i>Danio rerio</i>). Combining 16S rRNA high-throughput sequencing, micro-CT scan, metabolic parameters measurement, and gene expression analysis, we demonstrated that <i>B. licheniformis</i> FA6 changed the gut microbiota composition of zebrafish and increased both the Firmicutes/Bacteroidetes ratio and lipid accumulation. In terms of metabolites, <i>B. licheniformis</i> FA6 appeared to promote acetate pro2022-01-01T00:00:00Z2022 Dec2026-04-08T16:15:53.301Z2025-04-21T16:18:52.392ZS-EPMC98204763661411610.3390/ijms24010673