biostudies-literatureTamburino RItalian Ministry of Research (MiUR)Ministry of Education, Universities and Research309https://www.ebi.ac.uk/biostudies/studies/S-EPMC9820616biostudies-literatureUnknown24(1)Plant biomass is the most abundant renewable resource in nature. In a circular economy perspective, the implementation of its bioconversion into fermentable sugars is of great relevance. Lytic Polysaccharide MonoOxygenases (LPMOs) are accessory enzymes able to break recalcitrant polysaccharides, boosting biomass conversion and subsequently reducing costs. Among them, auxiliary activity of family 9 (AA9) acts on cellulose in synergism with traditional cellulolytic enzymes. Here, we report for the first time, the production of the AA9 LPMOs from the mesophilic <i>Trichoderma reesei</i> (TrAA9B) and the thermophilic <i>Thermoascus aurantiacus</i> (TaAA9B) microorganisms in tobacco by plastid transformation with the aim to test this technology as cheap and sustainable manufacture platform. In International journal of molecular sciencesTobacco Plastid Transformation as Production Platform of Lytic Polysaccharide MonoOxygenase Auxiliary Enzymes.PMC9820616PON03PE_00107_1-BioPoliS"PON03PE_00107_1-BioPoliS"Ionata ECastiglia DScotti NSannino LMarcolongo LTamburino RfalseTobacco Plastid Transformation as Production Platform of Lytic Polysaccharide MonoOxygenase Auxiliary Enzymes.Plant biomass is the most abundant renewable resource in nature. In a circular economy perspective, the implementation of its bioconversion into fermentable sugars is of great relevance. Lytic Polysaccharide MonoOxygenases (LPMOs) are accessory enzymes able to break recalcitrant polysaccharides, boosting biomass conversion and subsequently reducing costs. Among them, auxiliary activity of family 9 (AA9) acts on cellulose in synergism with traditional cellulolytic enzymes. Here, we report for the first time, the production of the AA9 LPMOs from the mesophilic <i>Trichoderma reesei</i> (TrAA9B) and the thermophilic <i>Thermoascus aurantiacus</i> (TaAA9B) microorganisms in tobacco by plastid transformation with the aim to test this technology as cheap and sustainable manufacture platform. In 2022-01-01T00:00:00Z2022 Dec2026-04-08T12:47:15.938Z2025-02-18T22:34:34.979ZS-EPMC98206163661375810.3390/ijms24010309