biostudies-literatureUnknown24(1)Tian YGlobal food security has benefited from the development and promotion of the two-line hybrid rice system. Excellent eating quality determines the market competitiveness of hybrid rice varieties based on achieving the fundamental requirements of high yield and good adaptability. Developing sterile and restorer lines with improved quality for two-line hybrid breeding by editing quality genes with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 is an efficient and practical alternative to the lengthy and laborious process of conventional breeding to improve rice quality. We edited <i>Wx</i> and <i>OsBADH2</i> using CRISPR/Cas9 technology to produce both homozygous male sterile mutant lines and homozygous restorer mutant lines with Cas9-free. These mutants have a much loInternational journal of molecular sciences849https://www.ebi.ac.uk/biostudies/studies/S-EPMC9820973biostudies-literatureCreation of Two-Line Fragrant Glutinous Hybrid Rice by Editing the <i>Wx</i> and <i>OsBADH2</i> Genes via the CRISPR/Cas9 System.PMC9820973Li YHe YTian YGao GLou GZhou YZhang QfalseCreation of Two-Line Fragrant Glutinous Hybrid Rice by Editing the <i>Wx</i> and <i>OsBADH2</i> Genes via the CRISPR/Cas9 System.Global food security has benefited from the development and promotion of the two-line hybrid rice system. Excellent eating quality determines the market competitiveness of hybrid rice varieties based on achieving the fundamental requirements of high yield and good adaptability. Developing sterile and restorer lines with improved quality for two-line hybrid breeding by editing quality genes with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 is an efficient and practical alternative to the lengthy and laborious process of conventional breeding to improve rice quality. We edited <i>Wx</i> and <i>OsBADH2</i> using CRISPR/Cas9 technology to produce both homozygous male sterile mutant lines and homozygous restorer mutant lines with Cas9-free. These mutants have a much lo2023-01-01T00:00:00Z2023 Jan2025-04-21T16:20:45.993Z2025-04-21T16:20:45.993ZS-EPMC98209733661429310.3390/ijms24010849