biostudies-literatureAmagai RGlaxoSmithKline JapanJapan Society for the Promotion of Science875https://www.ebi.ac.uk/biostudies/studies/S-EPMC9821026biostudies-literatureUnknown24(1)An active form of cathelicidin antimicrobial peptide, LL-37, has immunomodulatory and stimulatory effects, though the specific pathways are not clear. The purpose of this study was to identify the cellular pathways by which LL-37 amplifies the inflammation induced by damage-associated molecular patterns (DAMPs). We performed DNA microarray, reverse transcription polymerase chain reaction, immunoblotting, and proximity ligation assays using cultured keratinocytes treated with LL-37 and/or the DAMP poly(I:C), a synthetic double-stranded RNA. In contrast to the combination of LL-37 and poly(I:C), LL-37 alone induced genes related to biological metabolic processes such as VEGFA and PTGS2 (COX-2). Inhibition of FPR2, a known receptor for cathelicidin, partially suppressed the induction of VEGFAInternational journal of molecular sciencesThe Antimicrobial Peptide Cathelicidin Exerts Immunomodulatory Effects via Scavenger Receptors.PMC982102619K2125120K17304Research Grant 201918H06136Takahashi TFujimura TAsano YYamasaki KAiba SAmagai RTerui HfalseThe Antimicrobial Peptide Cathelicidin Exerts Immunomodulatory Effects via Scavenger Receptors.An active form of cathelicidin antimicrobial peptide, LL-37, has immunomodulatory and stimulatory effects, though the specific pathways are not clear. The purpose of this study was to identify the cellular pathways by which LL-37 amplifies the inflammation induced by damage-associated molecular patterns (DAMPs). We performed DNA microarray, reverse transcription polymerase chain reaction, immunoblotting, and proximity ligation assays using cultured keratinocytes treated with LL-37 and/or the DAMP poly(I:C), a synthetic double-stranded RNA. In contrast to the combination of LL-37 and poly(I:C), LL-37 alone induced genes related to biological metabolic processes such as VEGFA and PTGS2 (COX-2). Inhibition of FPR2, a known receptor for cathelicidin, partially suppressed the induction of VEGFA2023-01-01T00:00:00Z2023 Jan2025-04-21T16:18:08.1Z2025-04-21T16:18:08.1ZS-EPMC98210263661431310.3390/ijms24010875