biostudies-literatureUnknown12(1)Kishchenko OUniversity of HohenheimHuaiyin Normal UniversityNitrogen is an essential nutrient that affects all aspects of the growth, development and metabolic responses of plants. Here we investigated the influence of the two major sources of inorganic nitrogen, nitrate and ammonium, on the toxicity caused by excess of Mn in great duckweed, <i>Spirodela polyrhiza</i>. The revealed alleviating effect of ammonium on Mn-mediated toxicity, was complemented by detailed molecular, biochemical and evolutionary characterization of the species ammonium transporters (AMTs). Four genes encoding AMTs in <i>S. polyrhiza</i>, were classified as <i>SpAMT1;1</i>, <i>SpAMT1;2</i>, <i>SpAMT1;3</i> and <i>SpAMT2</i>. Functional testing of the expressed proteins in yeast and <i>Xenopus</i> oocytes clearly demonstrated activity of SpAMT1;1 and SpAMT1;3 in transportingPlants (Basel, Switzerland)208https://www.ebi.ac.uk/biostudies/studies/S-EPMC9824425biostudies-literatureAmmonium Uptake, Mediated by Ammonium Transporters, Mitigates Manganese Toxicity in Duckweed, <i>Spirodela polyrhiza</i>.PMC9824425Neuhauser BBorisjuk NStepanenko AStraub TZhou YKishchenko OfalseAmmonium Uptake, Mediated by Ammonium Transporters, Mitigates Manganese Toxicity in Duckweed, <i>Spirodela polyrhiza</i>.Nitrogen is an essential nutrient that affects all aspects of the growth, development and metabolic responses of plants. Here we investigated the influence of the two major sources of inorganic nitrogen, nitrate and ammonium, on the toxicity caused by excess of Mn in great duckweed, <i>Spirodela polyrhiza</i>. The revealed alleviating effect of ammonium on Mn-mediated toxicity, was complemented by detailed molecular, biochemical and evolutionary characterization of the species ammonium transporters (AMTs). Four genes encoding AMTs in <i>S. polyrhiza</i>, were classified as <i>SpAMT1;1</i>, <i>SpAMT1;2</i>, <i>SpAMT1;3</i> and <i>SpAMT2</i>. Functional testing of the expressed proteins in yeast and <i>Xenopus</i> oocytes clearly demonstrated activity of SpAMT1;1 and SpAMT1;3 in transporting2023-01-01T00:00:00Z2023 Jan2026-04-08T11:13:04.749Z2025-02-18T22:34:31.287ZS-EPMC98244253661633810.3390/plants12010208