biostudies-literatureMiller HENIH/NIACPRITNIA NIH HHSNIH/NCISU2C-CRUK Pediatric Cancer New Discoveries Challenge TeamDODNCI NIH HHSNIHNIGMS NIH HHSD1129-D1137https://www.ebi.ac.uk/biostudies/studies/S-EPMC9825527biostudies-literatureUnknown51(D1)R-loops are three-stranded nucleic acid structures formed from the hybridization of RNA and DNA. In 2012, Ginno et al. introduced the first R-loop mapping method. Since that time, dozens of R-loop mapping studies have been conducted, yielding hundreds of publicly available datasets. Current R-loop databases provide only limited access to these data. Moreover, no web tools for analyzing user-supplied R-loop datasets have yet been described. In our recent work, we reprocessed 810 R-loop mapping samples, building the largest R-loop data resource to date. We also defined R-loop consensus regions and developed a framework for R-loop data analysis. Now, we introduce RLBase, a user-friendly database that provides the capability to (i) explore hundreds of public R-loop mapping datasets, (ii) exploNucleic acids researchExploration and analysis of R-loop mapping data with RLBase.PMC9825527R01 CA152063R01CA152063GM139549CDMRP PR181598P30 CA054174RP150445R35 GM139549F31AG072902SU2C #RT6187R01 CA241554F31 AG0729021R01CA241554Hartono SLi JMiller HEMontemayor DBishop AJRFrost BPawar RLevy SASharma KChedin FfalseExploration and analysis of R-loop mapping data with RLBase.R-loops are three-stranded nucleic acid structures formed from the hybridization of RNA and DNA. In 2012, Ginno et al. introduced the first R-loop mapping method. Since that time, dozens of R-loop mapping studies have been conducted, yielding hundreds of publicly available datasets. Current R-loop databases provide only limited access to these data. Moreover, no web tools for analyzing user-supplied R-loop datasets have yet been described. In our recent work, we reprocessed 810 R-loop mapping samples, building the largest R-loop data resource to date. We also defined R-loop consensus regions and developed a framework for R-loop data analysis. Now, we introduce RLBase, a user-friendly database that provides the capability to (i) explore hundreds of public R-loop mapping datasets, (ii) explo2023-01-01T00:00:00Z2023 Jan2026-04-28T03:15:19.994Z2025-04-05T17:02:29.135ZS-EPMC98255273603975710.1093/nar/gkac732