biostudies-literatureAlcock BPNSERCDavid Braley Chair in Computational BiologyVisual and Automated Disease Analytics graduate training programCanadian Institutes of Health ResearchSimon Fraser UniversityMichael G. DeGroote Centre for Medicinal Cannabis ResearchCIHRCisco Research Chair in BioinformaticsMichael G. DeGroote Institute for Infectious Disease ResearchOntario Graduate Scholarship, McMaster University's MacDATA Institute Graduate FellowshipOntario Graduate ScholarshipFred and Helen Knight Enrichment AwardCanada Foundation for InnovationCIHR Canada Graduate ScholarshipOntario Research FundGenome CanadaOntario Graduate Scholarship, MacData Institute Graduate Fellowship, Ashbaugh Graduate ScholarshipMcMaster Service Lab and Repository computing clusterD690-D699https://www.ebi.ac.uk/biostudies/studies/S-EPMC9825576biostudies-literatureUnknown51(D1)The Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca) combines the Antibiotic Resistance Ontology (ARO) with curated AMR gene (ARG) sequences and resistance-conferring mutations to provide an informatics framework for annotation and interpretation of resistomes. As of version 3.2.4, CARD encompasses 6627 ontology terms, 5010 reference sequences, 1933 mutations, 3004 publications, and 5057 AMR detection models that can be used by the accompanying Resistance Gene Identifier (RGI) software to annotate genomic or metagenomic sequences. Focused curation enhancements since 2020 include expanded β-lactamase curation, incorporation of likelihood-based AMR mutations for Mycobacterium tuberculosis, addition of disinfectants and antiseptics plus their associated ARGs, and systematNucleic acids researchCARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database.PMC9825576FRN 143215PJT-159456RE09-047Smith KWFrench SLiang ZCJia BCarfrae LABhando TMaguire FDooley DEdalatmand AGolbon BBrown EDPetkau ATsang KKJiang XBrinkman FSLMukiri KMAlcock BPHuynh WMcCarthy MCKwong MShan PHsiao WWLNasir JABordeleau EVan Domselaar GBaker SJCChalil RMcArthur AGWlodarski MANiu KCYang JYJTu MMBeiko RGDave MHoad GRGray KLRachwalski KZubyk HLSyed SAKaur KRaphenya ARFarha MAGordzevich RImtiaz HGriffiths EfalseCARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database.The Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca) combines the Antibiotic Resistance Ontology (ARO) with curated AMR gene (ARG) sequences and resistance-conferring mutations to provide an informatics framework for annotation and interpretation of resistomes. As of version 3.2.4, CARD encompasses 6627 ontology terms, 5010 reference sequences, 1933 mutations, 3004 publications, and 5057 AMR detection models that can be used by the accompanying Resistance Gene Identifier (RGI) software to annotate genomic or metagenomic sequences. Focused curation enhancements since 2020 include expanded β-lactamase curation, incorporation of likelihood-based AMR mutations for Mycobacterium tuberculosis, addition of disinfectants and antiseptics plus their associated ARGs, and systemat2023-01-01T00:00:00Z2023 Jan2025-08-24T03:08:22.461Z2025-04-05T17:01:47.852ZS-EPMC98255763626382210.1093/nar/gkac920