biostudies-literatureTerlouw BRNatural Sciences and Engineering Council of Canada PGSDNatural Sciences and Engineering Council of CanadaCooperative Research Centres Projects schemeSECRETedPortuguese Science and Technology FoundationCARTNETNational Institutes of HealthConsejo Nacional de Ciencia y TecnologíaEuropean Research CouncilNCCIH NIH HHSDeutsche ForschungsgemeinschaftDutch Research Council (NWO)Novo Nordisk FondenNNF Center for BiosustainabilityBiotechnology and Biological Sciences Research CouncilDanish National Research FoundationUK government Department for Environment, Food and Rural AffairsNational Science FoundationNovo Nordisk FoundationGerman Chemical Industry scholarshipNetherlands eScience CenterHorizon 2020 Marie Skłodowska-Curie ActionsNIAID NIH HHSNational Research Foundation of KoreaHorizon 2020 Marie Sklodowska-Curie Individual FellowshipRothamsted Science Initiatives Catalyst AwardU.S. Department of EnergyLOEWE Center for Translational Biodiversity GenomicsFundaçao para a Ciencia e TecnologiaNetherlands Organization for Scientific Research (NWO) Veni ScienceUniversity of StrathclydeMARBLESNIGMS NIH HHSFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroOdo van Vloten foundationD603-D610https://www.ebi.ac.uk/biostudies/studies/S-EPMC9825592biostudies-literatureUnknown51(D1)With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role in the discovery of novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these pathways are often organised into biosynthetic gene clusters (BGCs). In 2015, we defined the Minimum Information about a Biosynthetic Gene cluster (MIBiG): a standardised data format that describes the minimally required information to uniquely characterise a BGC. We simultaneously constructed an accompanying online database of BGCs, which has since been widely used by the community as a reference dataset for BGCs and was expanded to 2021 entries in 2019 (MIBiG 2.0). Here, we describe MIBiG 3.0, a dNucleic acids researchMIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters.PMC9825592735867BBSRC948770101000392MSCA-IF-EF-ST-897121NNF10CC1016517757173U24 AT010811101000794765147SFRH/BD/136367/2018893122398967434-TRR 2611R01AI155694CRCPFIVE000119948770-DECIPHERF32 AT011475ASDI.2017.030U24AT010811F32 GM134688F32AT011475NRF-2020R1C1C1004046SFRH/BD/140567/2018R01 AI155694BB/R022054/1DNRF137Natural ProductsNNF20CC0035580DE-AC02-05CH11231NNF16OC0021746E-26/211.314/2019BB/W013959/1CBET-2032243GM134688NRF-2022R1C1C2004118VI.Veni.202.130Navarro-Munoz JCCruz-Morales PPourmohsenin BChagas FOHelfrich EJNKalkreuter ELoureiro CGutierrez-Garcia KCastelo-Branco RRex DABWitte TEKunjapur AMVind KAlanjary MWaschulin VChevrette MGZaroubi LBernaldo-Aguero JKalyvas NXie HAvitia-Dominguez LAAguilar CBooth TJVigneshwari AWilliams SEWinter JMPhilmus BPronk LJUBiermann FEgbert SMeijer DDuncan KRRoxborough ETYang DZhong ZKautsar SDu CUdwary DGayrard DCollemare JBlin KRecchia MJJLouwen JJRLee SBielinski VAvan der Hooft JJJAvelar-Rivas JAZdouc MYu JGavriilidou ASchorn MASingh KSAugustijn HELund GAleti Gvan Santen JARosas-Becerra LRAvalon NETerlouw BRCarrion Bravo VJBarona-Gomez FLi YXKang KBLin GMTang XSelem-Mojica NAl-Salihi SAAJati APHaslinger KRobinson SReitz ZLParra JScobie DJMedema MHRego AVromans SPJMKim WSokolova NWeber TTorring TLouwen NLLLinington RGfalseMIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters.With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role in the discovery of novel pharmaceutical drugs, crop protection agents and biomaterials. The genes that encode these pathways are often organised into biosynthetic gene clusters (BGCs). In 2015, we defined the Minimum Information about a Biosynthetic Gene cluster (MIBiG): a standardised data format that describes the minimally required information to uniquely characterise a BGC. We simultaneously constructed an accompanying online database of BGCs, which has since been widely used by the community as a reference dataset for BGCs and was expanded to 2021 entries in 2019 (MIBiG 2.0). Here, we describe MIBiG 3.0, a d2023-01-01T00:00:00Z2023 Jan2026-04-08T16:16:38Z2025-04-06T11:46:23.6ZS-EPMC98255923639949610.1093/nar/gkac1049