{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["52(10)"],"submitter":["Marsman C"],"pubmed_abstract":["Human naïve B cells are notoriously difficult to differentiate into antibody-secreting cells (ASCs) in vitro while maintaining sufficient cell numbers to evaluate the differentiation process. B cells require T follicular helper (T<sub>FH</sub> ) cell-derived signals like CD40L and IL-21 during germinal center (GC) responses to undergo differentiation into ASCs. Cognate interactions between B and T<sub>FH</sub> cells are transient; after T<sub>FH</sub> contact, B cells cycle between GC light and dark zones where T<sub>FH</sub> contact is present and absent, respectively. Here, we elucidated that the efficacy of naïve B cells in ACS differentiation is dramatically enhanced by the release of CD40L stimulation. Multiparameter phospho-flow and transcription factor (TF)-flow cytometry revealed t"],"journal":["European journal of immunology"],"pagination":["1662-1675"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9825913"],"repository":["biostudies-literature"],"pubmed_title":["Termination of CD40L co-stimulation promotes human B cell differentiation into antibody-secreting cells."],"pmcid":["PMC9825913"],"pubmed_authors":["Marsman C","Jorritsma T","Verstegen NJ","Ten Brinke A","van Ham SM","Boon L","Streutker M"],"additional_accession":[]},"is_claimable":false,"name":"Termination of CD40L co-stimulation promotes human B cell differentiation into antibody-secreting cells.","description":"Human naïve B cells are notoriously difficult to differentiate into antibody-secreting cells (ASCs) in vitro while maintaining sufficient cell numbers to evaluate the differentiation process. B cells require T follicular helper (T<sub>FH</sub> ) cell-derived signals like CD40L and IL-21 during germinal center (GC) responses to undergo differentiation into ASCs. Cognate interactions between B and T<sub>FH</sub> cells are transient; after T<sub>FH</sub> contact, B cells cycle between GC light and dark zones where T<sub>FH</sub> contact is present and absent, respectively. Here, we elucidated that the efficacy of naïve B cells in ACS differentiation is dramatically enhanced by the release of CD40L stimulation. Multiparameter phospho-flow and transcription factor (TF)-flow cytometry revealed t","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-21T14:19:52.261Z","creation":"2025-04-21T14:19:52.261Z"},"accession":"S-EPMC9825913","cross_references":{"pubmed":["36073009"],"doi":["10.1002/eji.202249972"]}}