{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["54(5)"],"submitter":["Fan G"],"pubmed_abstract":["PAC1-R is a recognized preferential receptor for the neuropeptide of pituitary adenylate cyclase-activating polypeptide (PACAP), which mediates neuroprotective and nerve regenerative activities of PACAP. In this study, we found that in both PAC1R-CHO cells with high expression of PAC1R-eGFP and retinal ganglion cells (RGC-5) with the natural expression of PAC1-R, oligo-peptide PACAP(28-38) and the positively charged arginine-rich penetrating peptide TAT, as positive allosteric modulators of PAC1-R, significantly trigger the nuclear translocation of PAC1-R. The chromatin immunoprecipitation (ChIP)-PCR results show that the nuclear translocated PAC1-R binds with the promoter regions of <i>PAC1-R</i> and its specific ligand <i>PACAP</i>. The up-regulated promoter activities of <i>PAC1-R</i> a"],"journal":["Acta biochimica et biophysica Sinica"],"pagination":["657-672"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9828401"],"repository":["biostudies-literature"],"pubmed_title":["Positive allosteric regulation of PAC1-R up-regulates PAC1-R and its specific ligand PACAP."],"pmcid":["PMC9828401"],"pubmed_authors":["Tao Z","Fan G","Yu R","Zhang H","Chen S"],"additional_accession":[]},"is_claimable":false,"name":"Positive allosteric regulation of PAC1-R up-regulates PAC1-R and its specific ligand PACAP.","description":"PAC1-R is a recognized preferential receptor for the neuropeptide of pituitary adenylate cyclase-activating polypeptide (PACAP), which mediates neuroprotective and nerve regenerative activities of PACAP. In this study, we found that in both PAC1R-CHO cells with high expression of PAC1R-eGFP and retinal ganglion cells (RGC-5) with the natural expression of PAC1-R, oligo-peptide PACAP(28-38) and the positively charged arginine-rich penetrating peptide TAT, as positive allosteric modulators of PAC1-R, significantly trigger the nuclear translocation of PAC1-R. The chromatin immunoprecipitation (ChIP)-PCR results show that the nuclear translocated PAC1-R binds with the promoter regions of <i>PAC1-R</i> and its specific ligand <i>PACAP</i>. The up-regulated promoter activities of <i>PAC1-R</i> a","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-19T04:46:19.177Z","creation":"2025-02-19T03:26:17.241Z"},"accession":"S-EPMC9828401","cross_references":{"pubmed":["35593471"],"doi":["10.3724/abbs.2022041"]}}