{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Barends TRM"],"funding":["NIGMS NIH HHS"],"pagination":["59"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9833121"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2"],"pubmed_abstract":["With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing time-resolved studies with unprecedented temporal resolution. This primer aims to assist structural biology groups with little or no experience in serial crystallography planning and carrying out a successful SFX experiment. It discusses the background of serial crystallography and its possibilities. Microcrystal growth and characterization methods are discussed, alongside techniques for sample delivery and data processing. Moreover, it gives p"],"journal":["Nature reviews. Methods primers"],"pubmed_title":["Serial femtosecond crystallography."],"pmcid":["PMC9833121"],"funding_grant_id":["R35 GM127086"],"pubmed_authors":["Cherezov V","Stauch B","Barends TRM","Schlichting I"],"additional_accession":[]},"is_claimable":false,"name":"Serial femtosecond crystallography.","description":"With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing time-resolved studies with unprecedented temporal resolution. This primer aims to assist structural biology groups with little or no experience in serial crystallography planning and carrying out a successful SFX experiment. It discusses the background of serial crystallography and its possibilities. Microcrystal growth and characterization methods are discussed, alongside techniques for sample delivery and data processing. Moreover, it gives p","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-26T12:54:45.599Z","creation":"2025-04-06T14:07:33.127Z"},"accession":"S-EPMC9833121","cross_references":{"pubmed":["36643971"],"doi":["10.1038/s43586-022-00141-7"]}}