{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["D'Amato G"],"funding":["NHLBI NIH HHS","NIGMS NIH HHS"],"pagination":["2517-2532.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9833645"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(22)"],"pubmed_abstract":["Endocardial cells lining the heart lumen are coronary vessel progenitors during embryogenesis. Re-igniting this developmental process in adults could regenerate blood vessels lost during cardiac injury, but this requires additional knowledge of molecular mechanisms. Here, we use mouse genetics and scRNA-seq to identify regulators of endocardial angiogenesis and precisely assess the role of CXCL12/CXCR4 signaling. Time-specific lineage tracing demonstrated that endocardial cells differentiated into coronary endothelial cells primarily at mid-gestation. A new mouse line reporting CXCR4 activity-along with cell-specific gene deletions-demonstrated it was specifically required for artery morphogenesis rather than angiogenesis. Integrating scRNA-seq data of endocardial-derived coronary vessels "],"journal":["Developmental cell"],"pubmed_title":["Endocardium-to-coronary artery differentiation during heart development and regeneration involves sequential roles of Bmp2 and Cxcl12/Cxcr4."],"pmcid":["PMC9833645"],"funding_grant_id":["T32 GM007276","R01 HL128503","T32 HL120824"],"pubmed_authors":["Rios Coronado PE","Quinn KE","Naftaly JA","Fan X","Amir ZA","Vigilante A","Cowley DO","Sharma B","D'Amato G","Phansalkar R","Caron KM","Red-Horse K"],"additional_accession":[]},"is_claimable":false,"name":"Endocardium-to-coronary artery differentiation during heart development and regeneration involves sequential roles of Bmp2 and Cxcl12/Cxcr4.","description":"Endocardial cells lining the heart lumen are coronary vessel progenitors during embryogenesis. Re-igniting this developmental process in adults could regenerate blood vessels lost during cardiac injury, but this requires additional knowledge of molecular mechanisms. Here, we use mouse genetics and scRNA-seq to identify regulators of endocardial angiogenesis and precisely assess the role of CXCL12/CXCR4 signaling. Time-specific lineage tracing demonstrated that endocardial cells differentiated into coronary endothelial cells primarily at mid-gestation. A new mouse line reporting CXCR4 activity-along with cell-specific gene deletions-demonstrated it was specifically required for artery morphogenesis rather than angiogenesis. Integrating scRNA-seq data of endocardial-derived coronary vessels ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T19:28:03.055Z","creation":"2025-04-04T19:28:03.055Z"},"accession":"S-EPMC9833645","cross_references":{"pubmed":["36347256"],"doi":["10.1016/j.devcel.2022.10.007"]}}