biostudies-literatureUnknown23(2)Ferreira VHMutations in the spike protein of SARS-CoV-2 have allowed Omicron subvariants to escape neutralizing antibodies. The degree to which this occurs in transplant recipients is poorly understood. We measured BA.4/5 cross-neutralizing responses in 75 mostly vaccinated transplant recipients who recovered from BA.1 infection. Sera were collected at 1 and 6 months post-BA.1 infection, and a lentivirus pseudovirus neutralization assay was performed using spike constructs corresponding to BA.1 and BA.4/5. Uninfected immunized transplant recipients and health care worker controls were used for comparison. Following BA.1 infection, the proportion of transplant recipients with neutralizing antibody responses was 88.0% (66/75) against BA.1 and 69.3% (52/75) against BA.4/5 (P = .005). The neutralization American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons278-283https://www.ebi.ac.uk/biostudies/studies/S-EPMC9835003biostudies-literatureImpact of Omicron BA.1 infection on BA.4/5 immunity in transplant recipients.PMC9835003Kumar DIerullo MKurtesi AFerreira VHHumar ASolera JTHu QGingras ACfalseImpact of Omicron BA.1 infection on BA.4/5 immunity in transplant recipients.Mutations in the spike protein of SARS-CoV-2 have allowed Omicron subvariants to escape neutralizing antibodies. The degree to which this occurs in transplant recipients is poorly understood. We measured BA.4/5 cross-neutralizing responses in 75 mostly vaccinated transplant recipients who recovered from BA.1 infection. Sera were collected at 1 and 6 months post-BA.1 infection, and a lentivirus pseudovirus neutralization assay was performed using spike constructs corresponding to BA.1 and BA.4/5. Uninfected immunized transplant recipients and health care worker controls were used for comparison. Following BA.1 infection, the proportion of transplant recipients with neutralizing antibody responses was 88.0% (66/75) against BA.1 and 69.3% (52/75) against BA.4/5 (P = .005). The neutralization 2023-01-01T00:00:00Z2023 Feb2025-04-05T13:22:03.385Z2025-02-19T01:27:17.07ZS-EPMC98350033674460610.1016/j.ajt.2022.10.004