biostudies-literatureShi JChinese Academy of Medical Science Clinical and Translational Medicine Research FoundationNational Natural Science Foundation of ChinaEmergency Key Program of Guangzhou LaboratoryCapital Health Development Scientific Research Project19https://www.ebi.ac.uk/biostudies/studies/S-EPMC9835228biostudies-literatureUnknown21(1)<h4>Background</h4>Due to the temporal and spatial heterogeneity of human epidermal growth factor receptor 2 (HER2) expression in breast tumors, immunohistochemistry (IHC) cannot accurately reflect the HER2 status in real time, which may cause misguided treatment decisions. HER2-specific imaging can noninvasively determine HER2 status in primary and metastatic tumors. In this study, HER2 expression in breast cancer patients was determined in vivo by SPECT/CT of ^99mTc-HP-Ark2, comparing with PET/CT of ¹⁸F-FDG lesion by lesion.<h4>Methods</h4>A novel HER2-targeted peptide probe ^99mTc-HP-Ark2 was constructed. Biodistribution and nanoScan SPECT/CT imaging were performed in mice models. The correlation between the quantified tumor uptake and HER2 expression in tJournal of translational medicineMolecular imaging of HER2 expression in breast cancer patients using a novel peptide-based tracer ^99mTc-HP-Ark2: a pilot study.PMC98352282018-1-4011819278028163004592159201EKPG21-16819716762019XK320032Luo QDu SZhu ZWang RWang FGao HShi JZhou YHou GfalseMolecular imaging of HER2 expression in breast cancer patients using a novel peptide-based tracer ^99mTc-HP-Ark2: a pilot study.<h4>Background</h4>Due to the temporal and spatial heterogeneity of human epidermal growth factor receptor 2 (HER2) expression in breast tumors, immunohistochemistry (IHC) cannot accurately reflect the HER2 status in real time, which may cause misguided treatment decisions. HER2-specific imaging can noninvasively determine HER2 status in primary and metastatic tumors. In this study, HER2 expression in breast cancer patients was determined in vivo by SPECT/CT of ^99mTc-HP-Ark2, comparing with PET/CT of ¹⁸F-FDG lesion by lesion.<h4>Methods</h4>A novel HER2-targeted peptide probe ^99mTc-HP-Ark2 was constructed. Biodistribution and nanoScan SPECT/CT imaging were performed in mice models. The correlation between the quantified tumor uptake and HER2 expression in t2023-01-01T00:00:00Z2023 Jan2026-05-28T06:11:43.086Z2025-02-19T02:22:02.482ZS-EPMC98352283663181210.1186/s12967-022-03865-y