{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Leong NKC"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","Croucher Foundation"],"pagination":["e13084"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9835441"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["<h4>Background</h4>Measures for mitigation of Coronavirus Disease 2019 (COVID-19) were set to reduce the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). SARS-CoV-2 and other respiratory viruses share similar transmission routes and some common clinical manifestations. Co-circulation of SARS-CoV-2 and other common respiratory viruses is imminent. Therefore, development of multiplex assays for detecting these respiratory viruses is essential for being prepared for future outbreaks of respiratory viruses.<h4>Methods</h4>A panel of three reverse transcription droplet digital PCR (RT-ddPCR) assays were developed to detect 15 different human respiratory viruses. Evaluations of its performance were demonstrated. A total of 100 local and 98 imported COVID-19 cases in Hong Kong were screened for co-infection with other common respiratory viruses.<h4>Results</h4>All detected viral targets showed distinct signal clusters using the multiplex RT-ddPCR assays. These assays have a broad range of linearity and good intra-/inter-assay reproducibility for each target. The lower limits of quantification for all targets were ≤46 copies per reaction. Six imported cases of COVID-19 were found to be co-infected with other respiratory viruses, whereas no local case of co-infection was observed.<h4>Conclusions</h4>The multiplex RT-ddPCR assays were demonstrated to be useful for screening of respiratory virus co-infections. The strict preventive measures applied in Hong Kong may be effective in limiting the circulation of other human respiratory viruses. The multiplex assays developed in this study can achieve a robust detection method for clinical and research purposes."],"journal":["Influenza and other respiratory viruses"],"pubmed_title":["Development of multiplex RT-ddPCR assays for detection of SARS-CoV-2 and other common respiratory virus infections."],"pmcid":["PMC9835441"],"funding_grant_id":["75N93021C00016"],"pubmed_authors":["Poon LLM","Gu H","Ng DYM","Chang LDJ","Krishnan P","Peiris M","Leong NKC","Cheng SSM"],"additional_accession":[]},"is_claimable":false,"name":"Development of multiplex RT-ddPCR assays for detection of SARS-CoV-2 and other common respiratory virus infections.","description":"<h4>Background</h4>Measures for mitigation of Coronavirus Disease 2019 (COVID-19) were set to reduce the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). SARS-CoV-2 and other respiratory viruses share similar transmission routes and some common clinical manifestations. Co-circulation of SARS-CoV-2 and other common respiratory viruses is imminent. Therefore, development of multiplex assays for detecting these respiratory viruses is essential for being prepared for future outbreaks of respiratory viruses.<h4>Methods</h4>A panel of three reverse transcription droplet digital PCR (RT-ddPCR) assays were developed to detect 15 different human respiratory viruses. Evaluations of its performance were demonstrated. A total of 100 local and 98 imported COVID-19 cases in Hong Kong were screened for co-infection with other common respiratory viruses.<h4>Results</h4>All detected viral targets showed distinct signal clusters using the multiplex RT-ddPCR assays. These assays have a broad range of linearity and good intra-/inter-assay reproducibility for each target. The lower limits of quantification for all targets were ≤46 copies per reaction. Six imported cases of COVID-19 were found to be co-infected with other respiratory viruses, whereas no local case of co-infection was observed.<h4>Conclusions</h4>The multiplex RT-ddPCR assays were demonstrated to be useful for screening of respiratory virus co-infections. The strict preventive measures applied in Hong Kong may be effective in limiting the circulation of other human respiratory viruses. The multiplex assays developed in this study can achieve a robust detection method for clinical and research purposes.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T13:07:33.164Z","creation":"2025-04-04T13:07:33.164Z"},"accession":"S-EPMC9835441","cross_references":{"pubmed":["36517993"],"doi":["10.1111/irv.13084"]}}