{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jethava KP"],"funding":["National Center for Advancing Translational Sciences","Purdue University Center for Cancer Research","NCATS NIH HHS","NCI NIH HHS","Eli Lilly and Company"],"pagination":["e202100378"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9835645"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(9)"],"pubmed_abstract":["Targeting live cell organelles is essential for imaging, understanding, and controlling specific biochemical processes. Typically, fluorescent probes with distinct structural scaffolds are used to target specific cell organelles. Here, we have designed a modular one-step synthetic strategy using a common reaction intermediate to develop new lysosomal, mitochondrial, and nucleus-targeting pH-activable fluorescent probes that are all based on a single boron dipyrromethane scaffold. The divergent cell organelle targeting was achieved by synthesizing probes with specific functional group changes to the central scaffold resulting in differential fluorescence and pK<sub>a</sub> . Specifically, we show that the functional group transformation of the same scaffold influences cellular localization "],"journal":["Chembiochem : a European journal of chemical biology"],"pubmed_title":["One Scaffold, Different Organelle Sensors: pH-Activable Fluorescent Probes for Targeting Live Microglial Cell Organelles."],"pmcid":["PMC9835645"],"funding_grant_id":["P30 CA023168","UL1 TR002529"],"pubmed_authors":["Prakash P","Jethava KP","Arora H","Chopra G","Manchanda P"],"additional_accession":[]},"is_claimable":false,"name":"One Scaffold, Different Organelle Sensors: pH-Activable Fluorescent Probes for Targeting Live Microglial Cell Organelles.","description":"Targeting live cell organelles is essential for imaging, understanding, and controlling specific biochemical processes. Typically, fluorescent probes with distinct structural scaffolds are used to target specific cell organelles. Here, we have designed a modular one-step synthetic strategy using a common reaction intermediate to develop new lysosomal, mitochondrial, and nucleus-targeting pH-activable fluorescent probes that are all based on a single boron dipyrromethane scaffold. The divergent cell organelle targeting was achieved by synthesizing probes with specific functional group changes to the central scaffold resulting in differential fluorescence and pK<sub>a</sub> . Specifically, we show that the functional group transformation of the same scaffold influences cellular localization ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-18T13:23:04.158Z","creation":"2025-04-06T23:01:59.34Z"},"accession":"S-EPMC9835645","cross_references":{"pubmed":["34585478"],"doi":["10.1002/cbic.202100378"]}}