{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dewey EB"],"funding":["National Cancer Institute","NCI NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["iyac164"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9836020"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["223(1)"],"pubmed_abstract":["Proper repair of DNA double-strand breaks is essential to the maintenance of genomic stability and avoidance of genetic disease. Organisms have many ways of repairing double-strand breaks, including the use of homologous sequences through homology-directed repair. While homology-directed repair is often error free, in single-strand annealing homologous repeats flanking a double-strand break are annealed to one another, leading to the deletion of one repeat and the intervening sequences. Studies in yeast have shown a relationship between the length of the repeat and single-strand annealing efficacy. We sought to determine the effects of homology length on single-strand annealing in Drosophila, as Drosophila uses a different annealing enzyme (Marcal1) than yeast. Using an in vivo single-stra"],"journal":["Genetics"],"pubmed_title":["The effect of repeat length on Marcal1-dependent single-strand annealing in Drosophila."],"pmcid":["PMC9836020"],"funding_grant_id":["R35 GM118127","1R35GM118127","T32 CA217824"],"pubmed_authors":["Saghaey K","Wittmer ME","Korda Holsclaw J","Dewey EB","Sekelsky J"],"additional_accession":[]},"is_claimable":false,"name":"The effect of repeat length on Marcal1-dependent single-strand annealing in Drosophila.","description":"Proper repair of DNA double-strand breaks is essential to the maintenance of genomic stability and avoidance of genetic disease. Organisms have many ways of repairing double-strand breaks, including the use of homologous sequences through homology-directed repair. While homology-directed repair is often error free, in single-strand annealing homologous repeats flanking a double-strand break are annealed to one another, leading to the deletion of one repeat and the intervening sequences. Studies in yeast have shown a relationship between the length of the repeat and single-strand annealing efficacy. We sought to determine the effects of homology length on single-strand annealing in Drosophila, as Drosophila uses a different annealing enzyme (Marcal1) than yeast. Using an in vivo single-stra","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-03-27T15:46:12.937Z","creation":"2025-04-06T13:10:04.284Z"},"accession":"S-EPMC9836020","cross_references":{"pubmed":["36303322"],"doi":["10.1093/genetics/iyac164"]}}