{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ravanfar R"],"funding":["NIDDK NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","Arnold and Mabel Beckman Foundation"],"pagination":["59-64"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9839481"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["597(1)"],"pubmed_abstract":["Flavocytochrome P450 from Bacillus megaterium (P450<sub>BM3</sub> ) is a natural fusion protein containing reductase and heme domains. In the presence of NADPH and dioxygen the enzyme catalyses the hydroxylation of long-chain fatty acids. Analysis of the P450<sub>BM3</sub> structure reveals chains of closely spaced tryptophan and tyrosine residues that might serve as pathways for high-potential oxidizing equivalents to escape from the heme active site when substrate oxidation is not possible. Our investigations of the total number of enzyme turnovers before deactivation have revealed that replacement of selected tryptophan and tyrosine residues with redox inactive groups leads to a twofold reduction in enzyme survival time. Tryptophan-96 is critical for prolonging enzyme activity, suggesting a key protective role for this residue."],"journal":["FEBS letters"],"pubmed_title":["Tryptophan-96 in cytochrome P450 BM3 plays a key role in enzyme survival."],"pmcid":["PMC9839481"],"funding_grant_id":["R01 DK019038","R01DK019038"],"pubmed_authors":["Sheng Y","Gray HB","Winkler JR","Ravanfar R"],"additional_accession":[]},"is_claimable":false,"name":"Tryptophan-96 in cytochrome P450 BM3 plays a key role in enzyme survival.","description":"Flavocytochrome P450 from Bacillus megaterium (P450<sub>BM3</sub> ) is a natural fusion protein containing reductase and heme domains. In the presence of NADPH and dioxygen the enzyme catalyses the hydroxylation of long-chain fatty acids. Analysis of the P450<sub>BM3</sub> structure reveals chains of closely spaced tryptophan and tyrosine residues that might serve as pathways for high-potential oxidizing equivalents to escape from the heme active site when substrate oxidation is not possible. Our investigations of the total number of enzyme turnovers before deactivation have revealed that replacement of selected tryptophan and tyrosine residues with redox inactive groups leads to a twofold reduction in enzyme survival time. Tryptophan-96 is critical for prolonging enzyme activity, suggesting a key protective role for this residue.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-29T14:11:39.135Z","creation":"2025-04-04T03:01:04.396Z"},"accession":"S-EPMC9839481","cross_references":{"pubmed":["36250256"],"doi":["10.1002/1873-3468.14514"]}}