{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["26(1)"],"submitter":["Xue W"],"pubmed_abstract":["The spinal cord possesses highly complex, finely organized cytoarchitecture guided by two dorsoventral morphogenic organizing centers. Thus, generation of human spinal cord tissue <i>in vitro</i> is challenging. Here, we demonstrated a novel method for generation of human dorsoventral spinal cord organoids using composite scaffolds. Specifically, the spinal cord ventralizing signaling Shh agonist (SAG) was loaded into a porous chitosan microsphere (PCSM), then thermosensitive Matrigel was coated on the surface to form composite microspheres with functional sustained-release SAG, termed as PCSM-Matrigel@SAG. Using PCSM-Matrigel@SAG as the core to induce 3D engineering of human spinal cord organoids from human pluripotent stem cells (ehSC-organoids), we found ehSC-organoids could form dorsov"],"journal":["iScience"],"pagination":["105898"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9840144"],"repository":["biostudies-literature"],"pubmed_title":["Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing."],"pmcid":["PMC9840144"],"pubmed_authors":["Liu H","Xue W","Li H","Ren L","Shao Z","Xiao Y","Li B"],"additional_accession":[]},"is_claimable":false,"name":"Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing.","description":"The spinal cord possesses highly complex, finely organized cytoarchitecture guided by two dorsoventral morphogenic organizing centers. Thus, generation of human spinal cord tissue <i>in vitro</i> is challenging. Here, we demonstrated a novel method for generation of human dorsoventral spinal cord organoids using composite scaffolds. Specifically, the spinal cord ventralizing signaling Shh agonist (SAG) was loaded into a porous chitosan microsphere (PCSM), then thermosensitive Matrigel was coated on the surface to form composite microspheres with functional sustained-release SAG, termed as PCSM-Matrigel@SAG. Using PCSM-Matrigel@SAG as the core to induce 3D engineering of human spinal cord organoids from human pluripotent stem cells (ehSC-organoids), we found ehSC-organoids could form dorsov","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-27T23:23:19.712Z","creation":"2024-12-03T22:58:59.662Z"},"accession":"S-EPMC9840144","cross_references":{"pubmed":["36647382"],"doi":["10.1016/j.isci.2022.105898"]}}