biostudies-literatureFilatava EJNINR NIH HHSNIH HHSU.S. Department of Health &amp; Human Services | National Institutes of Health60-67https://www.ebi.ac.uk/biostudies/studies/S-EPMC9840648biostudies-literatureUnknown43(1)<h4>Objective</h4>To compare pH of human milk types (mother's own milk (MOM), pasteurized donor human milk (PDHM), fortified MOM, and fortified PDHM) fed to preterm infants.<h4>Study design</h4>This observational study consisted of 63 mother-infant dyads < 34 weeks gestation. Human milk samples (n = 245), along with maternal factors, were collected for pH analysis. pH of MOM was analyzed over the course of lactation accounting for fortification status, postpartum day, and storage conditions.<h4>Results</h4>Mean pH of MOM was slightly acidic at 6.60 ± 0.28, which was significantly higher (p < 0.05) than other milk types. pH of MOM varied by fortification, postpartum day, and maternal vegetable/fiber intake. There was a significant interaction between fortification status and postpartum day; pH of MOM decreased over time, while pH of fortified MOM increased over time.<h4>Conclusion</h4>pH of human milk varied by type. pH of MOM was significantly associated with fortification status, postpartum day, and maternal vegetable/fiber intake.Journal of perinatology : official journal of the California Perinatal AssociationHuman milk pH is associated with fortification, postpartum day, and maternal dietary intake in preterm mother-infant dyads.PMC9840648R21NR017256R21 NR017256Gregas MGlynn RFilatava EJGregory KEOverton NEShelly CEfalseHuman milk pH is associated with fortification, postpartum day, and maternal dietary intake in preterm mother-infant dyads.<h4>Objective</h4>To compare pH of human milk types (mother's own milk (MOM), pasteurized donor human milk (PDHM), fortified MOM, and fortified PDHM) fed to preterm infants.<h4>Study design</h4>This observational study consisted of 63 mother-infant dyads < 34 weeks gestation. Human milk samples (n = 245), along with maternal factors, were collected for pH analysis. pH of MOM was analyzed over the course of lactation accounting for fortification status, postpartum day, and storage conditions.<h4>Results</h4>Mean pH of MOM was slightly acidic at 6.60 ± 0.28, which was significantly higher (p < 0.05) than other milk types. pH of MOM varied by fortification, postpartum day, and maternal vegetable/fiber intake. There was a significant interaction between fortification status and postpartum day; pH of MOM decreased over time, while pH of fortified MOM increased over time.<h4>Conclusion</h4>pH of human milk varied by type. pH of MOM was significantly associated with fortification status, postpartum day, and maternal vegetable/fiber intake.2023-01-01T00:00:00Z2023 Jan2025-04-04T21:13:37.138Z2025-04-04T21:13:37.138ZS-EPMC98406483597810510.1038/s41372-022-01492-5