{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Smith RJ"],"funding":["Bill and Melinda Gates Foundation","Wellcome Trust"],"pagination":["102002"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9841287"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(1)"],"pubmed_abstract":["Here, we provide a protocol using chemical pulldown combined with mass spectrometry (LC-MS/MS) to identify drug targets in Plasmodium falciparum. This approach works upon the principle that a resin-bound inhibitor selectively binds its molecular target(s) in cell-free lysates. We describe the preparation of drug beads and P. falciparum lysate, followed by chemical pulldown, sample fractionation, and LC-MS/MS analysis. We then detail how to identify specifically bound proteins by comparing protein enrichment in DMSO-treated relative to drug-treated lysates via quantitative proteomics. For complete details on the use and execution of this protocol, please refer to Milne et al. (2022).<sup>1</sup>."],"journal":["STAR protocols"],"pubmed_title":["Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysates."],"pmcid":["PMC9841287"],"funding_grant_id":["218448/Z/19/Z","105021/Z/14/Z","203134/Z/16/Z"],"pubmed_authors":["Dey G","Wyllie S","Lopez VC","Syed AJ","Milne R","Patterson S","Wiedemar N","Smith RJ"],"additional_accession":[]},"is_claimable":false,"name":"Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysates.","description":"Here, we provide a protocol using chemical pulldown combined with mass spectrometry (LC-MS/MS) to identify drug targets in Plasmodium falciparum. This approach works upon the principle that a resin-bound inhibitor selectively binds its molecular target(s) in cell-free lysates. We describe the preparation of drug beads and P. falciparum lysate, followed by chemical pulldown, sample fractionation, and LC-MS/MS analysis. We then detail how to identify specifically bound proteins by comparing protein enrichment in DMSO-treated relative to drug-treated lysates via quantitative proteomics. For complete details on the use and execution of this protocol, please refer to Milne et al. (2022).<sup>1</sup>.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-04-08T12:29:05.748Z","creation":"2025-02-19T03:27:33.456Z"},"accession":"S-EPMC9841287","cross_references":{"pubmed":["36609153"],"doi":["10.1016/j.xpro.2022.102002"]}}