{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bakr MN"],"funding":["Ministry of Higher Education"],"pagination":["101425"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9841360"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33"],"pubmed_abstract":["Nicotinic acetylcholine receptors (CHRNs) expression and their critical role in various types of cancer have been reported. However, it is still unclear which CHRNs and their associated genes play essential roles in metastasis in melanoma patients. Here, we performed bioinformatics analyses on publicly available bulk RNA sequencing (RNA-seq) data of patients with melanoma to identify the CHRNs highly expressed in metastatic melanoma. We found that <i>CHRNA1</i> was highly expressed in metastatic melanoma samples compared to primary melanoma samples and was strongly associated with <i>CHRNB1</i> and <i>CHRNG</i>. These muscle-type CHRNs (<i>CHRNA1</i>, <i>CHRNB1</i>, and <i>CHRNG</i>) were correlated with the <i>ZEB1</i> and Rho/ROCK pathway-related genes in metastatic melanoma samples. Pairwise correlations and enrichment analyses revealed that <i>CHRNA1</i> was significantly associated with myogenesis/muscle contraction and cell cycle genes. Kaplan-Meier curves illustrated the involvement of <i>CHRNA1</i>, four of its correlated genes (<i>DES</i>, <i>FLNC</i>, <i>CDK1</i>, and <i>CDC20</i>), and the myogenesis gene signature in the prognosis of melanoma patients. Following the bulk RNA-seq analysis, single-cell RNA-seq (scRNA-seq) analysis showed that the <i>CHRNA1</i>-expressing melanoma cells are primarily metastatic and had high expression levels of <i>CHRNB1</i>, <i>CHRNG</i>, and myogenesis/cell cycle-related genes. Our bioinformatics analyses of the bulk RNA-seq and scRNA-seq data of patients with melanoma revealed that <i>CHRNA1</i> and its correlated myogenesis/cell-related cycle genes are critical prognosis-related markers of metastatic melanoma."],"journal":["Biochemistry and biophysics reports"],"pubmed_title":["<i>CHRNA1</i> and its correlated-myogenesis/cell cycle genes are prognosis-related markers of metastatic melanoma."],"pmcid":["PMC9841360"],"funding_grant_id":["KH447"],"pubmed_authors":["Bakr MN","Kikuchi Y","Takahashi H"],"additional_accession":[]},"is_claimable":false,"name":"<i>CHRNA1</i> and its correlated-myogenesis/cell cycle genes are prognosis-related markers of metastatic melanoma.","description":"Nicotinic acetylcholine receptors (CHRNs) expression and their critical role in various types of cancer have been reported. However, it is still unclear which CHRNs and their associated genes play essential roles in metastasis in melanoma patients. Here, we performed bioinformatics analyses on publicly available bulk RNA sequencing (RNA-seq) data of patients with melanoma to identify the CHRNs highly expressed in metastatic melanoma. We found that <i>CHRNA1</i> was highly expressed in metastatic melanoma samples compared to primary melanoma samples and was strongly associated with <i>CHRNB1</i> and <i>CHRNG</i>. These muscle-type CHRNs (<i>CHRNA1</i>, <i>CHRNB1</i>, and <i>CHRNG</i>) were correlated with the <i>ZEB1</i> and Rho/ROCK pathway-related genes in metastatic melanoma samples. Pairwise correlations and enrichment analyses revealed that <i>CHRNA1</i> was significantly associated with myogenesis/muscle contraction and cell cycle genes. Kaplan-Meier curves illustrated the involvement of <i>CHRNA1</i>, four of its correlated genes (<i>DES</i>, <i>FLNC</i>, <i>CDK1</i>, and <i>CDC20</i>), and the myogenesis gene signature in the prognosis of melanoma patients. Following the bulk RNA-seq analysis, single-cell RNA-seq (scRNA-seq) analysis showed that the <i>CHRNA1</i>-expressing melanoma cells are primarily metastatic and had high expression levels of <i>CHRNB1</i>, <i>CHRNG</i>, and myogenesis/cell cycle-related genes. Our bioinformatics analyses of the bulk RNA-seq and scRNA-seq data of patients with melanoma revealed that <i>CHRNA1</i> and its correlated myogenesis/cell-related cycle genes are critical prognosis-related markers of metastatic melanoma.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-06-23T03:18:56.839Z","creation":"2025-02-19T03:27:36.141Z"},"accession":"S-EPMC9841360","cross_references":{"pubmed":["36654921"],"doi":["10.1016/j.bbrep.2023.101425"]}}