biostudies-literatureLim GMMinistry of Science and ICT, South KoreaNational Research Foundation of Korea419-430https://www.ebi.ac.uk/biostudies/studies/S-EPMC9841744biostudies-literatureUnknown31(6)There is a scarcity of experimental studies on peripheral nerve regeneration using placental extract (PE). This study aimed to investigate the effects of topical PE application on recovery after crush injury to the rat facial nerve using functional, electrophysiological, and morphological evaluations. The viability of the RSC96 Schwann cells treated with PE (0.5~4 mg/ml) increased significantly. Immunoblot test revealed that PE application enhanced the migration of RSC96 cells. Quantitative polymerase chain reaction demonstrated that PE increased the expression of neurotropic genes. The recovery from vibrissa fibrillation in the PE-treated group was superior to that in the control group. The threshold of action potential was also significantly lower in the PE group. Histopathological examiExperimental neurobiologyEnhancing the Effect of Placental Extract on the Regeneration of Crush Injured Facial Nerve.PMC98417442021R1A2C1014176Moon CJang CHCho GWChoi JHGanesan CDAng MJLim GMfalseEnhancing the Effect of Placental Extract on the Regeneration of Crush Injured Facial Nerve.There is a scarcity of experimental studies on peripheral nerve regeneration using placental extract (PE). This study aimed to investigate the effects of topical PE application on recovery after crush injury to the rat facial nerve using functional, electrophysiological, and morphological evaluations. The viability of the RSC96 Schwann cells treated with PE (0.5~4 mg/ml) increased significantly. Immunoblot test revealed that PE application enhanced the migration of RSC96 cells. Quantitative polymerase chain reaction demonstrated that PE increased the expression of neurotropic genes. The recovery from vibrissa fibrillation in the PE-treated group was superior to that in the control group. The threshold of action potential was also significantly lower in the PE group. Histopathological exami2022-01-01T00:00:00Z2022 Dec2025-04-04T13:36:10.069Z2025-04-04T13:36:10.069ZS-EPMC98417443663185010.5607/en22006