biostudies-literatureUnknown11Lin XOsteoarthritis (OA) is the most common joint disease in the world, characterized by pain and loss of joint function, which has led to a serious reduction in the quality of patients' lives. In this work, ultrahigh performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QToF/MS) in conjunction with multivariate pattern recognition methods and an univariate statistical analysis scheme were applied to explore the serum metabolic signatures within OA group (<i>n</i> = 31), HC (healthy controls) group (<i>n</i> = 57) and non-OA group (<i>n</i> = 19) for early diagnosis and differential diagnosis of OA. Based on logistic regression analysis and receiver operating characteristic (ROC) curve analysis, seven metabolites, including phosphatidylcholine (PeerJe14563https://www.ebi.ac.uk/biostudies/studies/S-EPMC9841907biostudies-literatureDiagnostic biomarker panels of osteoarthritis: UPLC-QToF/MS-based serum metabolic profiling.PMC9841907He SZhang TGong SWu SGao YLin XMinjie TfalseDiagnostic biomarker panels of osteoarthritis: UPLC-QToF/MS-based serum metabolic profiling.Osteoarthritis (OA) is the most common joint disease in the world, characterized by pain and loss of joint function, which has led to a serious reduction in the quality of patients' lives. In this work, ultrahigh performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QToF/MS) in conjunction with multivariate pattern recognition methods and an univariate statistical analysis scheme were applied to explore the serum metabolic signatures within OA group (<i>n</i> = 31), HC (healthy controls) group (<i>n</i> = 57) and non-OA group (<i>n</i> = 19) for early diagnosis and differential diagnosis of OA. Based on logistic regression analysis and receiver operating characteristic (ROC) curve analysis, seven metabolites, including phosphatidylcholine (2023-01-01T00:00:00Z20232025-04-04T14:28:26.226Z2025-04-04T14:28:26.226ZS-EPMC98419073665504310.7717/peerj.14563