biostudies-literatureZhang MNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaNatural Science Research of Jiangsu Higher Education Institutions of ChinaJiangsu Provincial Shuangchuang Doctor Talent Program26https://www.ebi.ac.uk/biostudies/studies/S-EPMC9844491biostudies-literatureUnknown8(1)Electrochemical oxidase biosensors have been widely applied in healthcare, environmental measurements and the biomedical field. However, the low and fluctuant oxygen levels in solution and the high anodic detection potentially restrict the assay accuracy. To address these problems, in this work, we constructed a three-phase interface enzyme electrode by sequentially immobilizing H₂O₂ electrocatalysts and an oxidase layer on a superhydrophobic laser-induced graphene (LIG) array substrate. The LIG-based enzyme electrode possesses a solid-liquid-air three-phase interface where constant and sufficient oxygen can be supplied from the air phase to the enzymatic reaction zone, which enhances and stabilizes the oxidase kinetics. We discovered that the enzymatic reaction rate Biomimetics (Basel, Switzerland)Laser-Induced Graphene Arrays-Based Three-Phase Interface Enzyme Electrode for Reliable Bioassays.PMC984449121KJB1500312210211121988102JSSCBS202107052019YFA070920022102111, 21988102Zhang JFeng XZhang MWang HHuang LDing ZfalseLaser-Induced Graphene Arrays-Based Three-Phase Interface Enzyme Electrode for Reliable Bioassays.Electrochemical oxidase biosensors have been widely applied in healthcare, environmental measurements and the biomedical field. However, the low and fluctuant oxygen levels in solution and the high anodic detection potentially restrict the assay accuracy. To address these problems, in this work, we constructed a three-phase interface enzyme electrode by sequentially immobilizing H₂O₂ electrocatalysts and an oxidase layer on a superhydrophobic laser-induced graphene (LIG) array substrate. The LIG-based enzyme electrode possesses a solid-liquid-air three-phase interface where constant and sufficient oxygen can be supplied from the air phase to the enzymatic reaction zone, which enhances and stabilizes the oxidase kinetics. We discovered that the enzymatic reaction rate 2023-01-01T00:00:00Z2023 Jan2025-04-05T12:56:25.189Z2025-04-05T12:56:25.189ZS-EPMC98444913664881210.3390/biomimetics8010026