biostudies-literatureFan AHNational Natural Science Foundation of ChinaNational Outstanding Youth Science Fund Project of National Natural Science Foundation of China513-524https://www.ebi.ac.uk/biostudies/studies/S-EPMC9844609biostudies-literatureUnknown12(1)Colorectal cancer (CRC) is a major leading cause of cancer mortality worldwide in which dysregulated protein synthesis plays an etiologic role. The eukaryotic elongation factor 1 A1 (eEF1A1) exerts significant effects on protein synthesis by contributing to peptide chain extension. Whereas its role in CRC remains to be investigated. In this study, we found that the mRNA and protein levels of eEF1A1 were significantly upregulated in CRC cell lines and tissues. Elevated expression of eEF1A1 was correlated with shorter overall survival in 94 CRC patients. The inhibition of proliferation and cell cycle block were observed in CRC cells after eEF1A1 downregulation. Mechanistically, weighted gene correlation network analysis and further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggCancer medicineeEF1A1 promotes colorectal cancer progression and predicts poor prognosis of patients.PMC984460981871913818220318197222482073197Liu HDuan LLi DZhang JLu YFan AHGuo TCheng HZhao XNie YFan DfalseeEF1A1 promotes colorectal cancer progression and predicts poor prognosis of patients.Colorectal cancer (CRC) is a major leading cause of cancer mortality worldwide in which dysregulated protein synthesis plays an etiologic role. The eukaryotic elongation factor 1 A1 (eEF1A1) exerts significant effects on protein synthesis by contributing to peptide chain extension. Whereas its role in CRC remains to be investigated. In this study, we found that the mRNA and protein levels of eEF1A1 were significantly upregulated in CRC cell lines and tissues. Elevated expression of eEF1A1 was correlated with shorter overall survival in 94 CRC patients. The inhibition of proliferation and cell cycle block were observed in CRC cells after eEF1A1 downregulation. Mechanistically, weighted gene correlation network analysis and further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis sugg2023-01-01T00:00:00Z2023 Jan2025-04-05T12:56:39.13Z2025-04-05T12:56:39.13ZS-EPMC98446093560794410.1002/cam4.4848